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HRP anti-His Tag Antibody

Pricing & Availability
Clone
J099B12 (See other available formats)
Regulatory Status
RUO
Other Names
Histidine Tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
J099B12_HRP_His-Tag_Antibody_1_061824
Whole cell extracts (15 µg total protein) from HEK293 cells either non-transfected or transfected with an expression vector for a His-tagged protein were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with HRP anti-His Tag (clone J099B12). Direct-Blot ™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • J099B12_HRP_His-Tag_Antibody_1_061824
    Whole cell extracts (15 µg total protein) from HEK293 cells either non-transfected or transfected with an expression vector for a His-tagged protein were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with HRP anti-His Tag (clone J099B12). Direct-Blot ™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • J099B12_HRP_His-Tag_Antibody_2_061824
    ELISA assay was performed by coating a 96-well plate with 100 µL/well of a His-tagged recombinant protein (100 ng/mL). The coated His-tagged recombinant protein was detected with HRP anti-His Tag Antibody (Clone J099B12) followed by TMB High Sensitivity Substrate Solution (Cat. No. 421501). Absorbance was measured at 450 nm.
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652503 25 µg £77
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652504 100 µg £282
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Description

This antibody was raised against linear 8XHis tag. It reacts with His tag protein, either C, N, or internal His6 and up repeats. It can be used for Western blotting to detect linear His repeat (His6 or more).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated His-tag peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solution with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light. Avoid exposure to sodium azide.
Application

WB - Quality tested
ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per mL. For direct ELISA, a concentration range of 0.001 - 0.1 µg per mL is recommended. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application References

(PubMed link indicates BioLegend citation)
  1. Burritt JB, et al. 1995. J. Biol. Chem. 270:16974.
  2. Taylor RM, et al. 2007. Methods Mol. Biol. 412:429.
Product Citations
  1. Bojadzic D, et al. 2021. Front Pharmacol. 11:600372. PubMed
  2. Konstantoulea K, et al. 2022. EMBO J. 41:e108591. PubMed
  3. Banta KL, et al. 2022. Immunity. 55:512. PubMed
  4. Zhang M, et al. 2022. Microbiol Spectr. 10:e0221221. PubMed
  5. Chuang ST, et al. 2022. Pharmaceuticals (Basel). 15: . PubMed
  6. Hay CE, et al. 2020. J Pharmacol Exp Ther. 16:374. PubMed
  7. Chen C, et al. 2023. Cell Biochem Funct. 41:355. PubMed
  8. Bojadzic D, et al. 2021. ACS Infect Dis. 7:1519. PubMed
  9. Wang HC, et al. 2020. Antimicrob Agents Chemother. 65:. PubMed
  10. Chen X, et al. 2021. Nat Commun. 12:5506. PubMed
  11. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  12. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  13. Zhang X, et al. 2021. Nat Chem. 13:626. PubMed
  14. Zhao Y, et al. 2019. Cancer Biol Ther. 0.925. PubMed
  15. Harrasser M, et al. 2022. Breast Cancer Res. 24:39. PubMed
  16. Chen X, et al. 2020. bioRxiv. . PubMed
  17. Houben B, et al. 2020. EMBO J. e102864:39. PubMed
  18. Hay CE, et al. 2018. PLoS One. 13:29. PubMed
RRID
AB_2563555 (BioLegend Cat. No. 652503)
AB_2563555 (BioLegend Cat. No. 652504)

Antigen Details

Function
Recombinant poly-histidine tag detection
Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about His Tag on UniProt.org
Go To Top Version: 4    Revision Date: 06/18/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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