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ELISA MAX™ Deluxe Set Human Adiponectin (Acrp30)

Pricing & Availability
Regulatory Status
RUO
Other Names
C1Q and collagen domain containing apM1, GBP28
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Product Citations
publications
Adiponectin_Human_ELISA_MAX_Deluxe_122915
  • Adiponectin_Human_ELISA_MAX_Deluxe_122915
Cat # Size Price Quantity Check Availability Save
442304 5 plates £238
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Description

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Adiponectin is also referred to as Acrp30, AdipoQ, apM1 and gelatin binding protein of 28 kD (GBP-28), which in humans is encoded by the ADIPOQ gene. Adiponectin plays an important role in carbohydrate and lipid metabolism. It promotes insulin sensitivity and protects cells from inflammation and apoptosis. Low serum levels of adiponectin are found in obesity, type 2 diabetes and coronary artery disease.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience, and performance of a high-end, commmercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing cost effectiveness, value, and ease of use.

The ELISA Max™ Set Deluxe is specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumathoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Adiponectin ELISA MAX™ Capture Antibody (200X)
  • Human Adiponectin ELISA MAX™ Detection Antibody (200X)
  • Human Adiponectin Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Human
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Itotagawa E, et al. 2023. Rheumatology (Oxford). 1988:62. PubMed
  2. Dumigan A, et al. 2022. EMBO Mol Med. e16888:14. PubMed
Sensitivity
31.3 pg/mL
Standard Range
62.5 - 4000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Soaline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCL, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20. BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (acid solution e.g. 2N H2SO4) BioLegend Cat. No. 423001
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent Paper

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 09/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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