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Human CD3/CD28 T Cell Activation Beads

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Regulatory Status
RUO
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A. Human_CD3_CD28_TCell_Activation_Beads_071823
Human CD3+ T cells were isolated from healthy human PBMCs using the MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480021). The CD3+ T cells were expanded with Human CD3/CD28 T Cell Activation Beads. At day 3 post-activation, the beads were removed using a MojoSort™ Magnet (Cat. No. 480019) while the cells remained in culture in the presence of IL-2 (Cat. No. 589102).
  • A. Human_CD3_CD28_TCell_Activation_Beads_071823
    Human CD3+ T cells were isolated from healthy human PBMCs using the MojoSort™ Human CD3 T Cell Isolation Kit (Cat. No. 480021). The CD3+ T cells were expanded with Human CD3/CD28 T Cell Activation Beads. At day 3 post-activation, the beads were removed using a MojoSort™ Magnet (Cat. No. 480019) while the cells remained in culture in the presence of IL-2 (Cat. No. 589102).
  • B. Human_CD3-CD28-T_Cell_Activation_Beads_2
    PD-1 expression at day 5 of culture of human CD3+ T cells stimulated with Human CD3/CD28 T Cell Activation Beads. At day of harvest, cells were stained with anti-human CD3 (clone SK7) APC and anti-human CD279 (PD-1) (clone NAT105) Brilliant Violet 421™ and were analyzed by flow cytometry. Cell debris, dead cells, and doublets were excluded from analysis.
  • c. 422603_011724.JPG
Cat # Size Price Quantity Check Availability Save
422603 0.5 mL £120
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422604 2.5 mL £421
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Description

The Human CD3/CD28 T Cell Activation Beads allow for convenient in vitro activation of human T cells without the need for antigen-presenting cells. Human T cells are stimulated with anti-CD3 and anti-CD28 antibodies coated on superparamagnetic microbeads. Simply add the beads directly to isolated T cells or to PBMCs at a 1:1 particle-to-cell ratio for robust T cell activation. The antibody-coated beads can be removed from the cells by the use of a magnetic separator. Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization. 

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat. No. 422603:

  • 1 vial containing 0.5 mL of Human CD3/CD28 T Cell Activation Beads

For Cat# 422604:

  • 1 vial containing 2.5 mL of Human CD3/CD28 T Cell Activation Beads

Product Details

Verified Reactivity
Human
Formulation
Cocktail: N/A

Particle: Phosphate buffer solution containing 0.1% HSA (azide-free). The particles are at a concentration of 4 x 107 particles/mL.

The Human CD3/CD28 T Cell Activation Beads are tested for microbial contamination.
Endotoxin Level
Less than 10 EU/mL as determined by the LAL test.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with magnetic microbeads under optimal conditions.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Recommended Usage

Use Human CD3/CD28 T Cell Activation Beads at a 1:1 particle-to-cell ratio.

Application Notes

This product is designed for the activation of human T cells.

Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in column: N/A

Antigen Details

Gene ID
NA
Go To Top Version: 1    Revision Date: 05/24/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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