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Human CD4 Treg Surface ID Panel

Pricing & Availability
Clone
A019D5; BC96; UCHT1; A161A1;
Regulatory Status
RUO
Other Names
Treg Panel
Isotype
Mouse IgG1, κ/Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Human_CD4_Treg_Surface_Panel_091724
Human PBMCs were stained using the Human CD4 Treg Surface ID Panel consisting of 7-AAD, anti-human CD3 APC/Cyanine7, CD4 FITC, CD25 APC, and CD127 (IL-7Rα) PE/Cyanine7 conjugated antibodies. Images represent live (7-ADD-) CD3+ cells (top panel), CD4+ cells (middle panel, histogram), and CD127 (IL-7Rα)lowCD25high Tregs. Cells were gated on lymphocytes.
  • Human_CD4_Treg_Surface_Panel_091724
    Human PBMCs were stained using the Human CD4 Treg Surface ID Panel consisting of 7-AAD, anti-human CD3 APC/Cyanine7, CD4 FITC, CD25 APC, and CD127 (IL-7Rα) PE/Cyanine7 conjugated antibodies. Images represent live (7-ADD-) CD3+ cells (top panel), CD4+ cells (middle panel, histogram), and CD127 (IL-7Rα)lowCD25high Tregs. Cells were gated on lymphocytes.
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362251 25 tests £342
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Description

This panel allows the identification of regulatory CD4 T cells (Treg) using surface markers, and it is intended to be applied to sort or analyze Treg cells using flow cytometry. The panel does not contain any conjugated PE antibody to leave room to incorporate an antibody of choice in the analysis, using a common and bright fluorophore. Tregs are critical to maintain immune system homeostasis by suppressing the function of other cells. Thus, they help maintain a proper balance between healthy and disease states such as autoimmunity and cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Formulation
Antibodies supplied in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
7-AAD supplied in PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
This reagent contains one vial each of 7-AAD, APC/Cyanine7 conjugated anti-human CD3, FITC conjugated anti-human CD4, APC conjugated anti-human CD25, and PE/Cyanine7 conjugated anti-human CD127 (IL-7Ra) at optimal concentration for multicolor flow cytometric analysis.

CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The reagent should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Application Notes

The panel has been verified by NanoString (https://www.nanostring.com) to be compatible with their Vantage 3D™ Immune Cell Profiling Assay for 3D Flow™ Analysis.

This product consists of four antibody vials, and one 7-AAD vial. Antibodies include APC/Cyanine7 anti-human CD3, FITC anti-human CD4, APC anti-human CD25, and PE/Cyanine7 anti-human CD127 (IL-7Rα). For a more flexible panel building design, please visit our Muticolor Panel Selector tool. If you prefer another live/dead reagent, please explore our chemical probes and Zombie dyes

RRID
AB_3097045 (BioLegend Cat. No. 362251)

Antigen Details

Cell Type
Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA 90:9125. 
  2. He YW, Malek TR. 1998. Crit. Rev. Immunol. 18:503. 
  3. Huster KM, et al. 2004. Proc. Natl. Acad. Sci. USA 101:5610.
  4. Taniguchi T, Minami Y. 1993. Cell 73:5. 
  5. Waldmann T. 1991. J. Biol. Chem. 266:2681.
  6. Beverley PC, Callard RE. 1981. Eur. J. Immunol. 11:329.
  7. Lanier LL, et al. 1986. J. Immunol. 137:2501-7.
  8. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
  9. Vignali DA. 2010. J. Immunol. 184:5933.
  10. Zhou L, et al. 2009. Immunity 30:646.
  11. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
  12. Zhu J, Paul WE. 2008. Blood 112:1557.
Gene ID
915 View all products for this Gene ID 920 View all products for this Gene ID 3559 View all products for this Gene ID 3575 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 2    Revision Date: 09/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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