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Human Naïve/Memory T cell ID Panel

Pricing & Availability
Clone
SK7; SK3; HI100; G043H7;
Regulatory Status
RUO
Other Names
Central memory, effector memory, TCM, TEM, CD3, CD4, CD45RA, CD197, CCR7
Isotype
Mouse IgG1, κ/Mouse IgG2a, κ/Mouse IgG2b, κ
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Product Citations
publications
Human_Naive_Memory_T_Cell_Panel_1_041024
Human peripheral blood lymphocytes were stained using the Human Naïve/Memory T cell ID Panel consisting of CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD45RA FITC and CD197 APC. Dot plots depict CD3+CD4+ T cells (left) and CD45RA+CCR7+ naïve T cells (TN), CD45RA-CCR7+ central memory T cells (TCM) and CD45RA-CCR7- effector memory T cells (TEM) (right, gated on CD4+ T cells).
  • Human_Naive_Memory_T_Cell_Panel_1_041024
    Human peripheral blood lymphocytes were stained using the Human Naïve/Memory T cell ID Panel consisting of CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD45RA FITC and CD197 APC. Dot plots depict CD3+CD4+ T cells (left) and CD45RA+CCR7+ naïve T cells (TN), CD45RA-CCR7+ central memory T cells (TCM) and CD45RA-CCR7- effector memory T cells (TEM) (right, gated on CD4+ T cells).
View NHP data of the APC format
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362201 50 tests £438
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Description

CD4+ T cells can be subdivided into naïve, memory, and effector subsets based on their expression of CD45RA and CCR7. Naïve T cells express high levels of CD45RA and CCR7, whereas memory T cells express variable levels of these markers. Expression of CCR7 and CD45RA allows differentiation of memory T cells into central (CCR7+CD45RA-) and effector memory (CCR7-CD45RA-) subsets. This panel of antibodies is designed for characterizing these three CD4+ T cell subsets, which may exhibit variations following antigenic stimulation such as viral infection or vaccination.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent contains one vial each of APC/Cyanine7 conjugated anti-CD3 (clone SK7), PerCP/Cyanine5.5 conjugated anti-CD4 (clone SK3), FITC conjugated anti-CD45RA (clone HI100), and APC conjugated anti-CD197 (clone G043H7) at optimal concentration for four-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product consists of four antibody vials: CD3 APC/Cyanine7, CD4 PerCP/Cyanine5.5, CD45RA FITC, and CD197 APC.

RRID
AB_3097691 (BioLegend Cat. No. 362201)

Antigen Details

Biology Area
Immunology
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverley P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
6. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
7. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
8. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
9. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
10. Reif K, et al. 2002. Nature 416:94.
11. Nakata B, et al. 2008. Oncology 74:69.

Gene ID
916 View all products for this Gene ID 920 View all products for this Gene ID 5788 View all products for this Gene ID 1236 View all products for this Gene ID
Go To Top Version: 3    Revision Date: 09/16/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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