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LDH-Cytox™ Assay Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lactate dehydrogenase assay
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Product Citations
publications
LDH_Cytox_Assay_Kit_Large_082117
Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
  • LDH_Cytox_Assay_Kit_Large_082117
    Human FASL induced cytotoxicity in Jurkat cells is measured using both LDH-Cytox™ Assay Kit (OD 490nm) and Deep Blue Cell Viability™ Kit (RFU x 103).
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426401 500 tests £157
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Description

LDH-Cytox Assay™ Kit is a kit for determination of cytotoxicity by measuring lactate dehydrogenase activity released from damaged cells. LDH catalyzes dehydrogenation of lactate to pyruvate thereby reducing NAD+ to NADH.  NADH reduces water-soluble tetrazolium salt in the presence of an electron mediator to produce an orange formazan dye.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Dye Mixture: Lyophilized, 1 bottle
  • Assay Buffer: 55 mL
  • Lysis Buffer: 5.5 mL
  • Stop Solution: 27.5 mL

Product Details

Formulation
This solution contains WST.
Application

Cyt - Quality tested
FA, BA - Reported in the literature, not verified in house
Recommended Usage

For more information, please refer to the product manual.

Application References

(PubMed link indicates BioLegend citation)
  1. Jin SF, et al. 2015. Exp Cell Res. 339(2):289-99.
  2. Watanabe S, et al. 2016. Biochem Biophys Res Commun. 471:191.
  3. Wu L, et al. 2015. Am J Physiol Gastrointest Liver Physiol. 309:G695.
  4. Fukami T, et al. 2016. Biochem. Pharmacol. 116:153-61.
  5. Tanaka M, et al. 2016. J Pharmacol Sci. 131(3):162-71.
  6. Wakatsuki S, et al. 2015. J Cell Biol. 211(4):881-96.
Product Citations
  1. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  2. Lyu Z, et al. 2021. Nat Biomed Eng. 5:847. PubMed
  3. Seyed-Khorrami SM, et al. 2021. Cancer Cell Int. 21:244. PubMed
  4. Hao Q, et al. 2022. Cells. 11:. PubMed
  5. Camp FA, et al. 2022. Cancers (Basel). 14:. PubMed
  6. Sauler M, et al. 2022. Nat Commun. 13:494. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Proliferation and Viability, Mitochondrial Function, Signal Transduction
Gene ID
NA

Related FAQs

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Go To Top Version: 3    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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