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LEAF™ Purified anti-human GDF-15 Antibody

Pricing & Availability
Clone
Poly5243 (See other available formats)
Regulatory Status
RUO
Other Names
MIC-1, NAG-1, pTGFB, PDF, PLAB
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5243_LEAF_GDF-15_Antibody_1_071723
Recombinant human GDF-15 (black circles) binds to immobilized recombinant GDNF receptor-alpha-like. LEAF™ Purified anti-human GDF-15 (Poly5243) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 1 ng/mL human GDF-15 to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 range is 0.016 – 0.1 µg/mL.
  • Poly5243_LEAF_GDF-15_Antibody_1_071723
    Recombinant human GDF-15 (black circles) binds to immobilized recombinant GDNF receptor-alpha-like. LEAF™ Purified anti-human GDF-15 (Poly5243) (purple squares) inhibits the binding in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. This antibody blocks the binding of 1 ng/mL human GDF-15 to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 range is 0.016 – 0.1 µg/mL.
  • Poly5243_LEAF_GDF-15_Antibody_2_071723
    Whole cell extracts (15 µg total protein) from HEL cells spiked with the indicated amount of recombinant human GDF-15 protein were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.125 µg/mL LEAF™ purified anti-human GDF-15 (clone Poly5243) for overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker.
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524303 100 µg £315
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Description

GDF-15 is a member of the TGF-β superfamily. GDF-15 is expressed as a 308 amino acids precursor that includes a signal peptide, a pro-domain, and a 112 residues mature protein. The secreted pro-domain binds to the extracellular matrix, where it can be activated by multiple enzymes such as furin (PCSK3), PCSK5, PCSK6, MMP-26, and MT1-MMP. GDF-15 is mainly expressed in placenta and prostate. Its expression is low in other tissues such as kidney, bladder, stomach, pancreas, liver, and gall bladder. GDF-15 is highly expressed in cancer cells with high levels detected in serum of cancer patients. GDF-15 has an important role in metabolic syndrome. GDF-15 transgenic mice present a lean phenotype. Furthermore, GDF-15 affects the macrophages in adipose tissue. Lean adipose tissue is characterized by the presence of anti-inflammatory M2-like macrophages. In obese individuals, adipose tissue show presence of CD8 effector cells and recruitment of inflammatory M1 macrophages. The increase of M1 macrophages over M2 results in metabolic changes, increase of TNFα, induction of glucose intolerance, and insulin insensitivity. GDF-15 binds to the GDNF receptor family member alpha like (GFRAL) which interacts with the co-receptor RET.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant Human GDF-15
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Blocking - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 1 ng/mL human GDF-15 to 0.1 µg/mL immobilized recombinant GDNF receptor-alpha-like. ND50 = 0.016 – 0.1 µg/mL. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068224 (BioLegend Cat. No. 524303)

Antigen Details

Structure
Homodimer
Distribution

Macrophages, adipocytes, cardiomyocytes, endothelial, and vascular smooth muscle cells. Expressed mainly in placenta and prostate.

Function
Regulates metabolism, appetite, cell survival, and immune tolerance. Regulated by androgens and calcitriol in prostate. Induced by inflammatory cytokines: IL-1b, TNFa, IL-2, and G-CSF.
Interaction
Neurons, gastric cancer cells, normal gastric mucosal cells, adipose tissue
Ligand/Receptor
GFRAL
Cell Type
Endothelial cells, Macrophages, Neurons
Biology Area
Cancer Biomarkers, Immuno-Oncology
Molecular Family
Growth Factors
Antigen References
  1. Bootcov MR, et al. 1997. Proc Natl Acad Sci USA. 94:1151.
  2. Emmerson PJ, et al. 2017. Nat Med. 23:1215.
  3. Li J, et al. 2018. Mol Cell Biol. 38: e00249.
  4. Li S, et al. 2014. J Clin Endocrinol Metab. 99: E2277.
  5. Nishimura S, et al. 2009. Nat Med. 15:914.
  6. Lackey DE, et al. 2016. Nat Rev Endocrinol. 12:15.
  7. Buchholz K, et al. 2021. J Cancer. 12:4698.
Gene ID
9518 View all products for this Gene ID
UniProt
View information about GDF-15 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/17/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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