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LEAF™ Purified anti-mouse IL-13 Antibody

Pricing & Availability
Clone
Poly5229 (See other available formats)
Regulatory Status
RUO
Other Names
P600, IL-13
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
A_Poly5229_LEAF_IL13_Antibody_061423
Recombinant mouse IL-13 (Cat. No. 575902) (black circles) induces proliferation on human TF-1 cells. LEAF™ purified anti-mouse IL-13 (clone Poly5229) (purple squares) neutralizes the cell proliferation induced by recombinant mouse IL-13 at 15 ng/mL in human TF-1 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range: 0.08 - 0.6 μg/mL.
  • A_Poly5229_LEAF_IL13_Antibody_061423
    Recombinant mouse IL-13 (Cat. No. 575902) (black circles) induces proliferation on human TF-1 cells. LEAF™ purified anti-mouse IL-13 (clone Poly5229) (purple squares) neutralizes the cell proliferation induced by recombinant mouse IL-13 at 15 ng/mL in human TF-1 cells in a dose-dependent manner, whereas the LEAF™ purified goat IgG isotype control (gray triangles) does not have the effect. ND50 range: 0.08 - 0.6 μg/mL.
  • B_Poly5229_LEAF_IL13_Antibody_WB_061423
    Whole cell extracts (15 µg total protein) from HeLa cells spiked with the indicated amount of recombinant mouse IL-13 protein (Cat. No. 575908) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL LEAF™ purified anti-mouse IL-13 (clone Poly5229) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker.
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522903 100 µg £312
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Description

Mouse IL-13 was initially cloned from cDNA libraries of activated T cells and was designated as P600. IL-13 is an immunoregulatory cytokine secreted predominantly by activated T(H)2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Rα). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. Interleukin-13 (IL-13), like IL-4, is a cytokine produced by T(H)2 type helper T cells in response to signaling through the T cell antigen receptor and by mast cells and basophils upon cross-linkage of the high-affinity receptor for immunoglobulin E (IgE). IL-13 has been implicated in airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse IL-13
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing proliferation induced by recombinant mouse IL-13 at 15 ng/mL in human TF-1 cells. ND50: 0.08 - 0.6 μg/mL. For western blotting, the suggested use of this reagent is 0.02 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3068100 (BioLegend Cat. No. 522903)

Antigen Details

Structure
Cytokine
Distribution

IL-13 is secreted by activated T cells, Th2 cells, basophils, mast cells, activated eosinophils and NK cells

Function
IL-13 modulates human monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in human monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β.
Interaction
IL-13 receptors are expressed on human B cells, basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells.
Ligand/Receptor
IL-4Rα, IL-13Rα1, and IL-13Rα2
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Brown KD, et al. 1989. J Immunol. 142:679-87.
  2. McKenzie AN, et al. 1993. Proc Natl Acad Sci U S A. 90:3735-9.
  3. Papasavvas E, et al. 2005. J Immunol. 175:5532-40.
  4. Kelly-Welch A, et al. Sci STKE. 293:2005.
  5. Hershey GK. 2003. J Allergy Clin Immunol. 111:677-90.
  6. Harris J, et al. 2007. Immunity. 27:505-17.
  7. LaPorte SL, et al. 2008. Cell. 132:259-72.
Gene ID
16163 View all products for this Gene ID
UniProt
View information about IL-13 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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