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LEAF™ Purified anti-mouse/rat TNF-α Antibody

Pricing & Availability
Clone
Poly5160 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5160_LEAF_TNF-a_Antibody_1_020821
Recombinant mouse TNF-α (Cat. No. 575206) (black circles) cytotoxicity on 929L mouse musculus cells. LEAF™ purified anti-mouse/rat TNF-α antibody (Poly5160) (purple squares) neutralizes the cytotoxicity function of recombinant mouse TNF-α (Cat. No. 575206) at 0.04 ng/mL on 929L cells in a dose-dependent manner whereas the GoInVivo™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.05 - 0.25 µg/mL.
  • Poly5160_LEAF_TNF-a_Antibody_1_020821
    Recombinant mouse TNF-α (Cat. No. 575206) (black circles) cytotoxicity on 929L mouse musculus cells. LEAF™ purified anti-mouse/rat TNF-α antibody (Poly5160) (purple squares) neutralizes the cytotoxicity function of recombinant mouse TNF-α (Cat. No. 575206) at 0.04 ng/mL on 929L cells in a dose-dependent manner whereas the GoInVivo™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 0.05 - 0.25 µg/mL.
  • Poly5160_LEAF_TNF-a_Antibody_2_020821
    Whole cell extracts (15 µg protein) from RAW264.7 cells that were pre-treated with 300 ng/mL brefeldin A solution (Cat. No. 420601) for 3 hours and unstimulated (-) or stimulated (+) with 100 ng/mL LPS for 6 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with a 1:5000 dilution of LEAF™ purified anti-mouse/rat TNF-α antibody (Poly5160) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat IgG antibody at a 1:10000 dilution. Equal protein loading was confirmed using HRP anti-β-actin antibody. Lane M: Molecular weight marker.
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516005 100 µg £301
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse TNF-α
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the cytotoxicity by recombinant mouse TNF-α (Cat. No. 575206) at 0.04 ng/mL on 929L mouse musculus cells. ND50 range: 0.05 - 0.25 µg/mL. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892477 (BioLegend Cat. No. 516005)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 18-150 kD (Mammalian).
Distribution

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.

Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
Cell Type
B cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 4    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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