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LEAF™ Purified anti-mouse SCF Antibody

Pricing & Availability
Clone
Poly5417 (See other available formats)
Regulatory Status
RUO
Other Names
Kit Ligand, KITLG, Mast cell growth factor, MGF, Steel factor, SLF, Stem Cell Factor
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5417_LEAF_SCF_Antibody_013123
Recombinant Mouse CD117-Fc chimera (Cat. No. 790802) (black circles) binds to immobilized Recombinant Mouse SCF (Cat. No. 579702). LEAF™ purified anti-mouse SCF (clone Poly5417) (purple squares) inhibits the binding in a dose-dependent manner, whereas LEAF™ purified goat IgG isotype control (gray triangles) does not have an effect. This antibody blocks the binding of 0.3 µg/mL Recombinant Mouse CD117-Fc chimera to 3 µg/mL immobilized Recombinant Mouse SCF. ND50 range: 0.07 - 0.6 µg/mL.
  • Poly5417_LEAF_SCF_Antibody_013123
    Recombinant Mouse CD117-Fc chimera (Cat. No. 790802) (black circles) binds to immobilized Recombinant Mouse SCF (Cat. No. 579702). LEAF™ purified anti-mouse SCF (clone Poly5417) (purple squares) inhibits the binding in a dose-dependent manner, whereas LEAF™ purified goat IgG isotype control (gray triangles) does not have an effect. This antibody blocks the binding of 0.3 µg/mL Recombinant Mouse CD117-Fc chimera to 3 µg/mL immobilized Recombinant Mouse SCF. ND50 range: 0.07 - 0.6 µg/mL.
  • Poly5417_LEAF_SCF_Antibody_WB_013123
    Whole cell extracts (15 µg total protein) from HeLa cells were spiked with or without the indicated amount of Recombinant Mouse SCF protein (Cat. No. 579702) and were resolved by 4-12% Bis-Tris gel electrophoresis, followed by being transferred to a PVDF membrane and probed with 0.5 µg/mL LEAF™ purified anti-mouse SCF (clone Poly5417) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP donkey anti-goat. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Lane M: Molecular weight marker
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541703 100 µg £247
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Description

SCF (KITL) is a hematopoietic growth factor, and it can synergize with a number of other cytokines to stimulate growth of hematopoietic progenitors in vitro and stimulates blood cell production in vivo. SCF is encoded by Sl ('steel'), a gene critical to the development of several distinct cell lineages during embryonic life. Even though KITL was identified as a soluble protein, the predicted amino acid sequence indicates that it is an integral transmembrane protein. KITL is generated by proteolytic cleavage from a transmembrane precursor. Two splice variants have been described for KITL, and proteolytic processing of both transmembrane protein products occurs on the cell surface. The binding of KITL induces the dimerization of the KIT molecule, followed by a change in the configuration of the intracellular domain and the autophosphorylation of the receptor, opening several docking sites for signal transduction molecules. Dysregulation of SCF–KI signaling and gain-of-function KIT mutations contribute to the genesis of many cancers, like acute myeloid leukemia and gastrointestinal stromal tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse SCF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Block - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.3 µg/mL recombinant mouse CD117-Fc chimera (Cat. No. 790802) to 3 µg/mL immobilized recombinant mouse SCF (Cat. No. 579702). ND50 range: 0.07 - 0.6 µg/mL. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody has not been tested for ICC.

RRID
AB_2936595 (BioLegend Cat. No. 541703)

Antigen Details

Structure
Cytokine
Distribution

SCF is widely expressed

Function
SCF has pleiotropic functions. It is involved in melanogenesis, gametogenesis, hematopoiesis, and brain angiogenesis.
Interaction
The KIT receptor tyrosine kinase (RTK) is expressed in hematopoietic stem cells (multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia cells.
Ligand/Receptor
SCF binds to the extracellular domains of the KIT receptor tyrosine kinase.
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Sun L, et al. 2006. Cancer Cell. 9:287-300.
  2. Magnol L, et al. 2007. BMC Dev Biol. 7:81.
  3. Liu H, et al. 2007. EMBO J. 26:891-901.
  4. Agosti V, et al. 2004. J Exp Med. 199:867-78.
  5. Huang E, el al. 1992. Mol Biol Cell. 3:349-62.
  6. Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
Gene ID
17311 View all products for this Gene ID
UniProt
View information about SCF on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01/31/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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