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- Regulatory Status
- RUO
- Other Names
- Microtubule-Associated Protein Tau, Phf-Tau, Paired Helical Filament-Tau, Neurofibrillary Tangle Protein, Microtubule-Associated Protein Tau, Isoform 4, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1
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- Submit a Review
- Product Citations
- publications
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Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Phospho-Tau (Thr217) standard ranging from 47 pg/mL to 3000 pg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 433107 | 1 Pre-coated Plate | £442 | ||||
Tau is a 441-amino acid protein that binds along microtubules in order to stabilize them. Phosphorylation of Tau reduces its affinity for microtubules, leading to microtubule destabilization. Hyperphosphorylated Tau can assemble into higher order structures, including oligomers and neurofibrillary tangles (NFT). NFTs are a hallmark of several neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and frontotemporal dementia. The level of phosphorylated Tau, therefore, is an important biomarker for the progression of these diseases. Phosphorylation of Tau at threonine 217 has become an important marker in Alzheimer's disease, for example, because it can be detected years ahead of typical disease symptoms and correlates well with disease severity.
The BioLegend LEGEND MAX™ Human/Mouse/Rat Phospho-Tau (Thr217) ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with mouse monoclonal Tau antibody. The detection antibody is a biotinylated rabbit polyclonal phospho-Tau (Thr217) antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. This kit is specifically designed for the accurate quantitation of phospho-Tau (Thr217) in human/mouse/rat brain lysates. This kit is analytically validated with ready-to-use reagents.
Kit Contents
- Kit Contents
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- Phospho-Tau (Thr217) Pre-coated 96-well Strip Microplate
- Phospho-Tau (Thr217) Detection Antibody
- Phospho-Tau (Thr217) Lyophilized Standard
- Streptavidin-Polymer HRP-M
- Assay Buffer B
- 20X Wash Buffer
- Substrate Solution F
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Application
-
ELISA
- Sensitivity
- 8.63 ± 1.71 pg/mL
- Standard Range
- 47 – 3000 pg/mL
- Materials Not Included
-
- Microplate reader able to measure absorbance at 450 nm
- Microplate shaker
- Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Polypropylene vials
Antigen Details
- Molecular Family
- Tau
- Gene ID
- 4137 View all products for this Gene ID
- UniProt
- View information about Phospho-Tau Thr217 on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
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We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
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The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
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In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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