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LEGEND MAX™ Mouse IL-21 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin 21, Za11, IL21 Precoated ELISA Kit
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Product Citations
publications
LEGENDMAX_Mouse_IL-21_ELISA_092817
  • LEGENDMAX_Mouse_IL-21_ELISA_092817
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446107 1 Pre-coated Plate £340
 
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Description

Interleukin 21 (IL-21) is a cytokine produced by CD4+ T cells, including T helper 17 (Th17) cells, T follicular helper (Tfh) cells, and natural killer T (NKT) cells. Functionally, IL-21 has pleiotropic effects on both innate and adaptive immune responses.  IL-21 enhances IFN? production and cytotoxicity in CD8+ T effector cells, and terminal differentiation in activated natural killer (NK) cells. Though not essential for the differentiation of Th17 cells in vitro or in vivo, IL-21 can drive Th17 responses in conjunction with TGF-ß. Production of IL-21 in CD4+ T cells is induced by IL-6 upon TCR stimulation; IL-6 and IL-21 are also key players in Tfh differentiation, driving increased expression of both bcl6 and CXCR5. In addition to its effects on T cells, IL-21 can also mediate cell proliferation, growth arrest, terminal differentiation, and apoptosis in normal B cells, depending on the target cell’s activation status. However, IL-21 has been reported to inhibit the proliferation of both human and murine B cells that have been stimulated with anti-IgM antibodies.

The BioLegend LEGEND MAX™ Mouse IL-21 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a polyclonal goat anti-mouse IL-21 capture antibody. The detection antibody is a biotinylated polyclonal goat anti-mouse IL-21 antibody. This kit is specifically designed for the accurate quantitation of mouse IL-21 from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Mouse IL-21 Pre-coated 96-well Strip Microplate
  • Mouse IL-21 Dectection Antibody
  • Mouse IL-21 Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Storage & Handling
Store unopened kit components between 2°C and 8°C.
Application

ELISA
Product Citations
  1. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
Sensitivity
1.39 pg/mL
Standard Range
4.7-300 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Th17, follicular helper T cells (TFH), Th2, memory CD4+ T cell, and NKT cells.
Biology Area
Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Gene ID
60505 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 09/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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