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LEGENDplex™ Mouse Macrophage/Microglia Panel Detection Antibodies

Pricing & Availability
Regulatory Status
RUO
Other Names
CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, IL-1β
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Cat # Size Price  
740851 100 tests (3.3 mL) £202
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To purchase, view our LEGENDplex™ Mix and Match page
Description

The LEGENDplex™ Mouse Macrophage/Microglia Panel Detection Antibodies contains 13 anti-mouse antibodies against CXCL1 (KC), Free Active TGF-β1, IL-18, IL-23, CCL22 (MDC), IL-10, IL-12p70, IL-6, TNF-α, G-CSF, CCL17 (TARC), IL-12p40, and IL-1β. This Detection cocktail is intended for use with the following in Mix and Match assays:

Mouse Macrophage/Microglia Panel

740852 LEGENDplex™ Mouse Macrophage/Microglia Panel Standard
740853 LEGENDplex™ Mouse CXCL1 (KC) Capture Bead A4, 13X
740854 LEGENDplex™ Mouse Free Active TGF-β1 Capture Bead A5, 13X
740855 LEGENDplex™ Mouse IL-18 Capture Bead A6, 13X
740856 LEGENDplex™ Mouse IL-23 Capture Bead A7, 13X
740857 LEGENDplex™ Mouse CCL22 (MDC) Capture Bead A8, 13X
740858 LEGENDplex™ Mouse IL-10 Capture Bead A10, 13X
740859 LEGENDplex™ Mouse IL-12p70 Capture Bead B2, 13X
740860 LEGENDplex™ Mouse IL-6 Capture Bead B3, 13X
740861 LEGENDplex™ Mouse TNF-α Capture Bead B4, 13X
740862 LEGENDplex™ Mouse G-CSF Capture Bead B5, 13X
740863 LEGENDplex™ Mouse CCL17 (TARC) Capture Bead B6, 13X
740864 LEGENDplex™ Mouse IL-12p40 Capture Bead B7, 13X
740865 LEGENDplex™ Mouse IL-1β Capture Bead B9, 13X
741446 LEGENDplex™ Buffer Set B1
740377 LEGENDplex™ Filter Plate
OR
740379 LEGENDplex™ V-Bottom Plate

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Application

Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
RRID
SCR_001134 (BioLegend Cat. No. 740851)

Antigen Details

Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
NA

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 3    Revision Date: 01/31/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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