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MojoSort™ Buffer (5X)

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Regulatory Status
RUO
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Product Citations
publications
MojoSortBuffer_1_051215
A single cell suspension from pooled mouse spleen and lymph nodes was processed with MojoSort™ Buffer as indicated in the protocol for isolation of CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit with the MojoSort™ Magnet. The purity was checked by staining with PE anti-mouse CD4 (clone RM4-4) and APC anti-mouse CD3ε (clone 145-2C11).
  • MojoSortBuffer_1_051215
    A single cell suspension from pooled mouse spleen and lymph nodes was processed with MojoSort™ Buffer as indicated in the protocol for isolation of CD4+ T cells using the MojoSort™ Mouse CD4 T Cell Isolation Kit with the MojoSort™ Magnet. The purity was checked by staining with PE anti-mouse CD4 (clone RM4-4) and APC anti-mouse CD3ε (clone 145-2C11).
  • MojoSortBuffer_2_051215
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480017 250 mL £46
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Description

MojoSort™ Buffer is formulated with high quality reagents to help reduce non-specific binding when using the MojoSort™ Magnetic Separation System.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
The buffer should be stored between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. The 5X concentrate is filtered through a 0.2 µm membrane. Use the buffer under aseptic conditions as needed.

Application Notes

MojoSort™ Buffer is recommended for cell separation using MojoSort™ nanobeads and kits. To use the buffer, follow the protocol provided with the MojoSort™ product you are using.

Composition:

  1. 5X Phosphate buffered saline (PBS), pH 7.2
  2. 2.5 % (w/v) Bovine Serum Albumin (BSA)
  3. 10 mM Ethylene Diamine Tetra-acetic acid (EDTA)
Product Citations
  1. Marié IJ, et al. 2022. Bio Protoc. 12:e4438. PubMed
  2. Guo H, et al. 2022. Biomaterials. 283:121441. PubMed
  3. Knittel J, et al. 2022. STAR Protoc. 3:101632. PubMed
  4. Woodring T, et al. 2022. iScience. 25:104934. PubMed
  5. He C, et al. 2021. Immunol Cell Biol. 99:611. PubMed
  6. Lauko A, et al. 2022. Cell Rep. 40:111348. PubMed
  7. Xie L, et al. 2020. Infect Immun. :88. PubMed

Antigen Details

Gene ID
NA

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05/14/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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