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MojoSort™ Human CD14 Selection Kit

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Regulatory Status
RUO
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Product Citations
publications
CD14_MojoSort_CD14_SelectionKit_1_111615
PBMCs were prepared to isolate CD14+ cells using the MojoSort™ Human CD14 Selection Kit. Cells were stained with CD14 PE (clone HCD14). Dead cells were excluded by 7-AAD.
  • CD14_MojoSort_CD14_SelectionKit_1_111615
    PBMCs were prepared to isolate CD14+ cells using the MojoSort™ Human CD14 Selection Kit. Cells were stained with CD14 PE (clone HCD14). Dead cells were excluded by 7-AAD.
  • CD14_MojoSort_CD14_SelectionKit_2_111615
Cat # Size Price Quantity Check Availability Save
480025 20 tests £159
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480026 200 tests £507
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Description

CD14+ cells are either selected or depleted by incubating your sample with the biotin conjugated anti-human CD14 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD14+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. CD14 is expressed at high levels on human monocytes and macrophages and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cell, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in pathogen clearance and upregulation of adhesion molecules and cytokine expression. Clone 63D3 has been reported not to block LPS induced signal.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480025:

  • 1 vial containing 200 µl of biotin anti-human CD14 antibody (clone 63D3)
  • 1 vial containing 200 µl Streptavidin Nanobeads


For Cat# 480026

  • 2 vials containing 1 ml each of biotin anti-human CD14 antibody (clone 63D3)
  • 2 vials containing 1 ml each of Streptavidin Nanobeads

Product Details

Verified Reactivity
Human
Formulation
Antibody: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2 with stabilizer.

Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.

Storage & Handling
Antibody and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of antibody for 1x107 cells in 100 µl of buffer.

10 µl Streptavidin Nanobeads for 1x107 cells in 100 µl of buffer.

Application Notes

The expected purity is 90% CD14+ cells post-separation of human peripheral blood mononuclear cells from healthy donors, analyzed by flow cytometry

This kit is designed for the positive selection or depletion of CD14+ cells from peripheral blood mononuclear cells (PBMCs).

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Antibody or cocktail dilution to use in column: 16X
Nanobead dilution to use in columns: 3X

Product Citations
  1. Xiao R, et al. 2022. Nat Commun. 13:4661. PubMed

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Gene ID
NA
UniProt
View information about CD14 on UniProt.org

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 05/25/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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