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MojoSort™ Human CD45 Selection Kit

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Mojosort_Human_CD45_Selection_Kit_051223
A single-cell suspension mixture of 30% human peripheral blood mononuclear cells (PBMCs) with 70% human colorectal adenocarcinoma cell line HT-29 was prepared for positive selection of CD45+ cells using the MojoSort™ Human CD45 Selection Kit. Cells were stained with APC anti-human CD326 (clone CO17-1A) (Cat. No. 369809) and a cocktail of PE conjugated antibodies against CD3/CD14/CD15/CD19/CD56 (clones UCHT1/HCD14/W6D3/HIB19/HCD56) (Cat. Nos. 300407/325605/323005/302207/318305). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305).
  • Mojosort_Human_CD45_Selection_Kit_051223
    A single-cell suspension mixture of 30% human peripheral blood mononuclear cells (PBMCs) with 70% human colorectal adenocarcinoma cell line HT-29 was prepared for positive selection of CD45+ cells using the MojoSort™ Human CD45 Selection Kit. Cells were stained with APC anti-human CD326 (clone CO17-1A) (Cat. No. 369809) and a cocktail of PE conjugated antibodies against CD3/CD14/CD15/CD19/CD56 (clones UCHT1/HCD14/W6D3/HIB19/HCD56) (Cat. Nos. 300407/325605/323005/302207/318305). Dead cells were excluded by Helix NP™ Blue (Cat. No. 425305).
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480167 10 tests £136
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480168 100 tests £407
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Description

Human CD45+ expressing cells are either selected or depleted by incubating the sample with anti-human CD45 Biotin Antibody followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD45+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat. No. 480167:

  • 1 vial containing 100 µL of anti-human CD45 Biotin-Antibody
  • 1 vial containing 100 µL of Streptavidin Nanobeads

For Cat. No. 480168:

  • 1 vial containing 1 mL of anti-human CD45 Biotin-Antibody
  • 1 vial containing 1 mL of Streptavidin Nanobeads​

Product Details

Verified Reactivity
Human
Formulation
Cocktail: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Particle: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
All components should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µL of anti-human CD45 Biotin-Antibody for 1 x 107 cells in 100 µL of buffer.
10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer.

Application Notes

This kit is designed for the positive selection or depletion of CD45+ leukocytes from human tissues or tumors. 

Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Antibody or cocktail dilution to use in column: 4X
Nanobead dilution to use in column: 4X

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05/12/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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