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MojoSort™ Human CD8 Memory T Cell Isolation Kit

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MojoSort_Human_CD8_Memory_TCell_Isolation_Kit_1_101524
PBMCs were prepared to isolate CD8+ Memory T cells using the MojoSort™ Human CD8 Memory T Cell Isolation Kit. The first dot plot shows T cells after isolation stained with CD8 (clone SK1) Brilliant Violet 421™ and CD3 (clone UCHT1) APC/Cyanine7. The second plot shows memory T cells after isolation, defined by CD45RO (clone UCHL1) PE and CD45RA (clone HI100) APC. Events shown were not previously gated on CD8+CD3+ cells. The third dot plot shows CD8+CD3+ cells before isolation. Dead cells were excluded by 7-AAD.
  • MojoSort_Human_CD8_Memory_TCell_Isolation_Kit_1_101524
    PBMCs were prepared to isolate CD8+ Memory T cells using the MojoSort™ Human CD8 Memory T Cell Isolation Kit. The first dot plot shows T cells after isolation stained with CD8 (clone SK1) Brilliant Violet 421™ and CD3 (clone UCHT1) APC/Cyanine7. The second plot shows memory T cells after isolation, defined by CD45RO (clone UCHL1) PE and CD45RA (clone HI100) APC. Events shown were not previously gated on CD8+CD3+ cells. The third dot plot shows CD8+CD3+ cells before isolation. Dead cells were excluded by 7-AAD.
  • MojoSort_Human_CD8_Memory_TCell_Isolation_Kit_2_101524
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD8+ Memory T cells using the MojoSort™ Human CD8 Memory T cell Isolation Kit. Cells after isolation (Top, Left) and Cells before isolation (Bottom, Left), stained with CD8 (clone: SK1) BV421 and CD3 (clone: UCHT1) APC/Cyanine7. Cells after isolation (Top, Middle) and Cells before isolation (Bottom, Right) stained with CD45RO (clone: UCHL1) PE and CD45RA (clone: HI100) APC, gated on CD8+CD3+ from (Top, Left) and (Bottom, Left), respectively. CD45RO and CD45RA profile of isolated cells (Top, Right) analyzed directly without CD8+CD3+ parent gate. Dead cells were excluded by 7-AAD.
Cat # Size Price Quantity Check Availability Save
480065 20 tests £177
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480066 200 tests £570
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Description

CD8 Memory T cells are depleted by incubating the sample with the Human CD8 Memory T cell isolation biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched human CD8 Memory T cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents

For Cat# 480065:

  • 200 µl Biotin-Antibody Cocktail
  • 200 µL Streptavidin Nanobeads

For Cat# 480066:

  • 2 vials of 1 ml of Biotin-Antibody Cocktail each
  • 2 vials of 1 ml Streptavidin Nanobeads each

Product Details

Verified Reactivity
Human
Formulation
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.

Application Notes

This kit is designed for the isolation of untouched CD8 Memory T cells from peripheral blood mononuclear cells (PBMCs).
 

Antibody or cocktail dilution to use in column: 6X
Nanobead dilution to use in columns: 2X

Antigen Details

Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
NA

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 2    Revision Date: 01/26/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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