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ELISA MAX™ Deluxe Set Mouse CCL8 (MCP-2)

Pricing & Availability
Regulatory Status
RUO
Other Names
Monocyte chemotactic protein 2 (MCP-2), small inducible cytokine subfamily A, member 8 (SCYA8),Chemokine (C-C motif) ligand 8, CCL8, MCP2, MCP-2
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Product Citations
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MU_CCL8_MCP2_ELISAMAX_Deluxe_120718
  • MU_CCL8_MCP2_ELISAMAX_Deluxe_120718
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446904 5 plates £209
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Description

Note: This product does not include ELISA microwell plates. Cat. No. 423501 is recommended.

CCL8, also known as MCP-2, is a CC chemokine of the monocyte chemoattractant protein (MCP) family that plays key roles in allergic and inflammatory responses. CCL8 (MCP-2) has structural and functional similarity to other family members MCP-1 and MCP-3, and is often co-expressed with these chemokines in mononuclear cells. CCL8 is also constitutively expressed in the skin and lymph nodes of mice. Mouse CCL8 induces a chemotactic migration of a population of differentiated Th2 cells enriched in IL-5 and IL-25 receptors by binding to the CCR8 receptor. Recent studies have established CCL8 as a critical ligand for driving CCR8-dependent Th2 cell trafficking into the skin, which in conjunction with locally recruited eosinophils drives chronic cutaneous allergic inflammation. CCL8 expression by suprabasal buldge cells has also been experimentally shown to negatively regulate the population of mouse hair follicles by Langerhans cells, further underscoring this chemokines central contributions to maintaining cutaneous immunity.

The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The mouse (CCL8 MCP-2) capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and CCL8 (MCP-2) binds to the immobilized capture antibody. Next, biotinylated anti-mouse CCL8 (MCP-2) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of CCL8 (MCP-2) present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse CCL8 (MCP-2) ELISA MAX™ Capture Antibody (200X)
  • Mouse CCL8 (MCP-2) ELISA MAX™ Detection Antibody (200X)
  • Mouse CCL8 (MCP-2) Standard
  • Avidin-HRP (1000X)
  • Substrate Solution C
  • Coating Buffer B (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Product Citations
  1. Lin CH, et al. 2023. iScience. 26:106904. PubMed
Sensitivity
5.1 ± 3.3 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Epithelial cells, Leukocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
20307 View all products for this Gene ID
UniProt
View information about CCL8 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 3    Revision Date: 02/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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