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Mouse MDSC Flow Cocktail 2 with Isotype Ctrl

Pricing & Availability
Clone
M1/70; 1A8; HK1.4;
Regulatory Status
RUO
Other Names
Myeloid derived suppressor cells flow kit, CD11b/Ly6G/Ly6C
Isotype
Rat IgG2a, κ/Rat IgG2b, κ/Rat IgG2c, κ
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Product Citations
publications
MDSC_cocktail_2_Mouse_Antibodies_FC_1_012914
C57BL/6 mouse splenocytes were stained with mouse MDSC Flow Cocktail 2 (CD11b PE/Ly-6G FITC/Ly-6C APC).
  • MDSC_cocktail_2_Mouse_Antibodies_FC_1_012914
    C57BL/6 mouse splenocytes were stained with mouse MDSC Flow Cocktail 2 (CD11b PE/Ly-6G FITC/Ly-6C APC).
  • MDSC_cocktail_2_Mouse_Antibodies_FC_2_012914
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147003 25 tests £238
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Description

This mouse MDSC Flow Cocktail 2 is composed of CD11b, Ly-6C, and Ly-6G antibodies. MDSCs are a heterogenous population of cells that can affect T cell function. Two different subsets of MDSC exist: CD11b+Ly-6G+Ly-6Clow (granulocytic MDSC) and CD11b+Ly-6G-Ly-6Chi (monocytic MDSC). These cells have been identified in a variety of pathological conditions including cancer and autoimmune disease.

CD11b: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Ly-6G: Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Ly-6C: Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a multicolor cocktail of PE conjugated CD11b (clone M1/70), FITC conjugated Ly-6G (clone 1A8) and APC conjugated Ly-6C (clone HK1.4) at optimal concentration for three-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Materials provided:

1. Mouse MDSC Flow Cocktail (containing CD11b PE/Ly-6G FITC/Ly-6C APC), 25 tests
2. Mouse MDSC Isotype Control Cocktail (containing rat IgG2b PE/rat IgG2a FITC/rat IgG2c APC), 25 tests

Materials not provided:

Cell Staining Buffer (Cat. No. 420201)
Single color compensation controls

Product Citations
  1. Feng Q, et al. 2023. JCI Insight. 8:. PubMed
  2. Clayton KL, et al. 2018. Nat Immunol. 475:19. PubMed
  3. Xie L, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
RRID
AB_3097688 (BioLegend Cat. No. 147003)

Antigen Details

Biology Area
Immunology
Antigen References

1. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1.
2. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205.
4. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
5. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
6. Cerwenka A, et al. 1998. J. Immunol. 161:97.
7. Springer TA. 1994. Cell. 76:301.
8. Coxon A, et al. 1996. Immunity 5:653.
9. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505.
10. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151.

Gene ID
16409 View all products for this Gene ID 17067 View all products for this Gene ID 3684 View all products for this Gene ID 546644 View all products for this Gene ID
Go To Top Version: 2    Revision Date: 12/03/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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