- Clone
- 6D5; 53-7.3; 1B1;
- Regulatory Status
- RUO
- Other Names
- Breg, B regulatory
- Isotype
- Rat IgG2a/Rat IgG2b
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_B10FlowKit_050109.jpg A_B10FlowKit_050109.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A_B10FlowKit_050109.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells. -
C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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132601 | 25 tests | £238 |
Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. BioLegend's Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.
Product DetailsKit Contents
- Kit Contents
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- FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor® 647 anti-mouse CD1d, 25 tests
- FITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor® 647 Rat IgG2b isotype controls, 25 tests
Product Details
- Verified Reactivity
- Mouse
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
-
Upon receipt, store between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product. - Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells.
- RRID
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AB_2073522 (BioLegend Cat. No. 132601)
- Materials Not Included
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- Cell Staining Buffer (Cat. No. 420201)
- TruStain FcX (Cat. No. 101319)
- Single color compensation controls
Antigen Details
- Distribution
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Represent 1%-2% of spleen B cells in wild-type mice, but expand to about 10% of spleen B cells in hCD19Tg mice.
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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Yanaba K, et al. 2008. Immunity 25:639.
- Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Related FAQs
Other Formats
View All CD19 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Mouse Regulatory B Cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647) | 6D5; 53-7.3; 1B1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Mouse Regulatory B Cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647)
C57BL/6 mouse splenocytes were stained with Mouse Regulatory... C57BL/6 mouse splenocytes were stained with Mouse Regulatory...
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