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Pacific Blue™ anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
MMA (See other available formats)
Regulatory Status
RUO
Other Names
FUT4, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1, 3-FAL, 3-FL, LNFP III, X-hapten
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
MMA_PB_CD15_Antibody_091218
Human peripheral blood granulocytes were stained with Pacific Blue™ CD15 (clone MMA, filled histogram) or Pacific Blue™ Mouse IgM, κ isotype control (open histogram).
  • MMA_PB_CD15_Antibody_091218
    Human peripheral blood granulocytes were stained with Pacific Blue™ CD15 (clone MMA, filled histogram) or Pacific Blue™ Mouse IgM, κ isotype control (open histogram).
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394703 25 tests £97
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394704 100 tests £201
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
U-937 human cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

In-house testing suggests that clone MMA does not block W6D3 also raised against human CD15 (SSEA-1)

Application References

(PubMed link indicates BioLegend citation)
  1. Hanjan S.N, et al. 1982. Clin. Immunol. and Immunopathol. 23(2):172 (FC)
  2. Greil R, et al. 2018. Oncotarget. 9(29):20928 (IHC)
  3. Brcic L, et al. 2018. Virchows. Arch. 472(4):589 (IHC/FC)
  4. Karai B, et al. 2018. Biochem. Med. 28(2):020704 (FC)
  5. Chan RC, et al. 2018. Biomed Rep. 8(3):224 (FC)
Product Citations
  1. Castellano E, et al. 2022. Front Immunol. 13:970931. PubMed
RRID
AB_2750479 (BioLegend Cat. No. 394703)
AB_2750479 (BioLegend Cat. No. 394704)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Phagocytosis and chemotaxis
Ligand/Receptor
CD62E
Cell Type
Neutrophils, Eosinophils, Monocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
Go To Top Version: 1    Revision Date: 09/13/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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