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Pacific Blue™ anti-human CD294 (CRTH2) Antibody

Pricing & Availability
Clone
BM16 (See other available formats)
Regulatory Status
RUO
Other Names
Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM16_PB_CD294_Antibody_071118
Human peripheral blood lymphocytes were stained with CD194 (CCR4) PE, and CD294 (clone BM16) Pacific Blue™ (left) or rat IgG2a, κ Pacific Blue™ isotype control (right).
  • BM16_PB_CD294_Antibody_071118
    Human peripheral blood lymphocytes were stained with CD194 (CCR4) PE, and CD294 (clone BM16) Pacific Blue™ (left) or rat IgG2a, κ Pacific Blue™ isotype control (right).
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350129 25 tests £81
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350130 100 tests £209
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Description

CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat cell line TART/B19-12.10 transfected with human CRTH2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

This product is sold under license agreement with BML, Inc for research use only.

Application References

(PubMed link indicates BioLegend citation)
  1. Nagata K, et al. 1999. J. Immunol. 162:1278. (FC)
  2. De Fanis U, et al. 2007. Blood 109:4343. (FC)
  3. Tsuda H, et al. 2001. Clin. Exp. Immunol. 123:105. (FC)
  4. Hamada K, et al. 2004. Allergology International 53:179. (FC)
Product Citations
  1. Ferrer-Font L, et al. 2021. Curr Protoc. 1:e222. PubMed
RRID
AB_2750056 (BioLegend Cat. No. 350129)
AB_2750056 (BioLegend Cat. No. 350130)

Antigen Details

Structure
Seven-transmembrane protein coupled with G proteins
Distribution

Effector T helper 2 (Th2) cells, eosinophils, basophils

Function
Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Interaction
Heterotrimeric G proteins
Ligand/Receptor
Prostaglandin D2
Cell Type
Th2, Eosinophils, Basophils
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
CD Molecules
Antigen References

1. Luster AD and Tager AM. 2004. Nat. Rev. Immunol. 4:711.
2. Monneret G, et al. 2001. Blood 98:1942.
3. Xue L, et al. 2009. J. Immunol. 182:7580.
4. Schratl P, et al. 2007. J. Immunol. 179:4792.

Gene ID
11251 View all products for this Gene ID
UniProt
View information about CD294 on UniProt.org
Go To Top Version: 1    Revision Date: 07/11/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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