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Pacific Blue™ anti-mouse/human Helios Antibody

Pricing & Availability
Clone
22F6 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype
Armenian Hamster IgG
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Product Citations
publications
22F6_PB_1_060310
Human peripheral blood lymphocytes surface stained with CD4-PE (RPA-T4), and then were intracellulary stained with Helios-Pacific Blue™ 647 (clone 22F6).
  • 22F6_PB_1_060310
    Human peripheral blood lymphocytes surface stained with CD4-PE (RPA-T4), and then were intracellulary stained with Helios-Pacific Blue™ 647 (clone 22F6).
  • 22F6_PB_2_060310
    C57BL/6 splenocytes surface stained with CD4-PE (GK1.5), and then were intracellularly stained with Helios-Pacific Blue™ (clone 22F6).
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137210 25 tests £145
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137220 100 tests £286
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Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Helios peptide (aa 51-107)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol.
For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
  2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
  3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
  4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
  5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
  6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
  7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations
  1. Danileviciute E, et al. 2022. Nat Metab. 4:589. PubMed
  2. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  3. Schmitt E, et al. 2013. J Immunol. 191:5640. PubMed
  4. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  5. Schoenbrunn A, et al. 2012. J Immunol. 189:5985. PubMed
  6. Hope C, et al. 2014. Kidney Int. 86:175. PubMed
  7. Merwe M, et al. 2013. J Immunol. 191:5746. PubMed
  8. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  9. Kreiser S, et al. 2014. Immunobiology. 220:270. PubMed
  10. Singh K, et al. 2015. Sci Rep. 14:7767. PubMed
  11. Bouvy A, et al. 2014. Nephrol Dial Transplant. 29:1587. PubMed
  12. Yero A, et al. 2019. J Virol. 93:e00371-19. PubMed
  13. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  14. Esfahani K, et al. 2019. Nat Commun. 3.688888889. PubMed
  15. Baptista A, et al. 2014. Elife. 3:4433. PubMed
  16. Watson MJ, et al. 2021. Nature. 591:645. PubMed
  17. Dhuban K, et al. 2015. J Immunol. 194:3687. PubMed
  18. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  19. Hu M, et al. 2019. Nat Commun. 10:3031. PubMed
  20. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  21. Serre K, et al. 2011. PLoS One. 6:e20731. PubMed
  22. Yang YH, et al. 2020. Cell Reports. 30(5):1515-1529.e4. PubMed
  23. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  24. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  25. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  26. Chopp LB, et al. 2020. Immunity. 53(6):1182-1201.e8. PubMed
  27. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  28. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_10575625 (BioLegend Cat. No. 137210)
AB_10575625 (BioLegend Cat. No. 137220)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al.  1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org
Go To Top Version: 2    Revision Date: 02/03/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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