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PE anti-Apo E Antibody

Pricing & Availability
Clone
E6D7 (See other available formats)
Regulatory Status
RUO
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
E6D7_PE_ApoE_Antibody_030419.png
Human PBMC monocytes were cultured overnight with (left) or without (right) 100ng/mL recombinant human TGF-β1 followed by a 4 hour addition of 1X Brefeldin A (left) or DMSO (right). Cells were then fixed with Fixation Buffer (BioLegend Cat. No. 420801) and permeabilzed with Intracellular Staining Permeabilization Wash Buffer (10X) (BioLegend Cat. No. 421002). Cells were stained with CD11b (clone ICRF44) APC and anti-ApoE Antibody (clone E6D7) PE.
  • E6D7_PE_ApoE_Antibody_030419.png
    Human PBMC monocytes were cultured overnight with (left) or without (right) 100ng/mL recombinant human TGF-β1 followed by a 4 hour addition of 1X Brefeldin A (left) or DMSO (right). Cells were then fixed with Fixation Buffer (BioLegend Cat. No. 420801) and permeabilzed with Intracellular Staining Permeabilization Wash Buffer (10X) (BioLegend Cat. No. 421002). Cells were stained with CD11b (clone ICRF44) APC and anti-ApoE Antibody (clone E6D7) PE.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
803404 25 tests £105
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803405 100 tests £247
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of Apo-E.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody is effective in immunoblotting (WB).

Clone E6D7 recognizes the E2, E3, and E4 isoforms of apolipoprotein E.

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG, Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics 4(2):101-108.
  3. Saunders AM. 2000. J. Neuropathol. Exp. Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys. J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res. Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin. Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol. Life Sci. 56(3-4):268-79.
  8. Golabek AA., et al. 1999. J. Biol. Chem. 271(18):10602-6.
RRID
AB_2801140 (BioLegend Cat. No. 803404)
AB_2801140 (BioLegend Cat. No. 803405)

Antigen Details

Cell Type
Astrocytes, Mature Neurons, Microglia, Monocytes, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance
Molecular Family
APP/Aβ Degradation, Apolipoproteins
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/04/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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