PE anti-β Catenin 1 Antibody, beta Catenin 1, 15B8

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Clone: 15B8 (See other available formats)
Regulatory Status: RUO
Other Names: β-catenin, CTNNB1
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human colon adenocarcinoma cell line SW480 treated (open historgram) or untreated (filled histogram) with tankyrase inhibitor XAV939 for 18 hours, then treated with True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) and stained with anti-β Catenin 1 (clone 15B8) PE.

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862603

25 tests

£117

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862604

100 tests

£263

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Description

β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2832862 (BioLegend Cat. No. 862603)
AB_2832862 (BioLegend Cat. No. 862604)

Antigen Details

Structure: β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
Distribution: Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular
Function: β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
Interaction: E-cadherin/CDH1, LEF1, TCF4, etc.
Antigen References: Dias C, et al. 2014. Nature. 516(7529): 51

Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3

Na K, et al. 2017. Anticancer Res. 37(6):3249

Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
Gene ID: 1499 View all products for this Gene ID 12387 View all products for this Gene ID 84353 View all products for this Gene ID
UniProt: View information about beta Catenin 1 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All β Catenin 1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-β Catenin 1	15B8	WB,IHC-P,ICFC
PE anti-β Catenin 1	15B8	ICFC
PE/Cyanine7 anti-β Catenin 1	15B8	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 02/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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