- Clone
- FIT-22 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fluorescein isothiocyanate
- Isotype
- Mouse IgG1, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
408307 | 25 µg | £42 | ||||
408308 | 100 µg | £92 |
The FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- FITC conjugated mouse IgG.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_2783325 (BioLegend Cat. No. 408307)
AB_2783325 (BioLegend Cat. No. 408308)
Antigen Details
- Gene ID
- NA
- UniProt
- View information about FITC on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All FITC Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-FITC | FIT-22 | FC |
Biotin anti-FITC | FIT-22 | FC |
Purified anti-FITC (Maxpar® Ready) | FIT-22 | FC,CyTOF® |
APC anti-FITC | FIT-22 | FC |
PE anti-FITC | FIT-22 | FC |
TotalSeq™-C0988 anti-FITC | FIT-22 | PG |
TotalSeq™-A0988 anti-FITC | FIT-22 | PG |
TotalSeq™-B0988 anti-FITC | FIT-22 | PG |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-FITC
C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4... C57BL/6 thymocytes stained with RM4-5 FITC only (top) or RM4... -
Biotin anti-FITC
-
Purified anti-FITC (Maxpar® Ready)
Human PBMC stained with FITC-anti-CD3 (UCHT1) then detected ... -
APC anti-FITC
C57BL/6 thymocytes stained with anti-CD4 (clone RM4-5) FITC ... -
PE anti-FITC
C57BL/6 thymocytes stained with CD4 (clone RM4-5) FITC only ... -
TotalSeq™-C0988 anti-FITC
-
TotalSeq™-A0988 anti-FITC
-
TotalSeq™-B0988 anti-FITC
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