PE anti-HMGB1 Antibody anti-HMGB1

Pricing & Availability

Clone: 3E8 (See other available formats)
Regulatory Status: RUO
Other Names: High mobility group protein 1, High mobility group protein B1, Sulfoglucuronyl carbohydrate binding protein
Isotype: Mouse IgG2b, κ
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Product Citations: publications

3E8_PE_HMGB1_Antibody_FC_061013 — Hela cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with anti-HMGB1 (clone 3E8) PE (filled histogram) or mouse IgG2b, κ PE isotype control (open histogram).

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651403

25 tests

£97

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651404

100 tests

£238

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Description

High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1.

HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human HMGB1 with GST tag. A universal T cell epitope from a Mycobacterium tuberculosis antigen was introduced into the C-terminus of HMGB1 to increase the immunogenicity.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone 3E8 has been reported to protect mice from LPS induced sepsis¹. Additional reported applications (for relevant formats) include: neutralization¹. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
RRID: AB_2562220 (BioLegend Cat. No. 651403)
AB_2562220 (BioLegend Cat. No. 651404)

Antigen Details

Structure: 215 amino acids with predicted molecular weight of 25 kD.
Distribution: Nucleus
Function: DNA binding protein that associates with chromatin and has the ability to bend DNA. Binds single-stranded DNA preferentially. Involved in V(D)J recombination by acting as a cofactor of the RAG complex.
Interaction: Component of the RAG complex composed of core components RAG1 and RAG2.
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thomas JO and Travers AA. 2001. Trends Biochem. Sci. 26:167.
2. Müller S, et al. 2004. J. Intern. Med. 255:332.
3. Campana L, et al. 2008. Curr. Opin. Immunol. 20:518.
4. Klune JR, et al. 2008. Mol. Med. 14:476.
5. Dumitriu IE, et al. 2005. Trends Immunol. 26:381.
6. Bonaldi T, et al. 2003. EMBO J. 22:5551.
Gene ID: 3146 View all products for this Gene ID
UniProt: View information about HMGB1 on UniProt.org

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All HMGB1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-HMGB1	3E8	WB,ICC,IHC-P
PE anti-HMGB1	3E8	ICFC
Alexa Fluor® 594 anti-HMGB1	3E8	ICC
Alexa Fluor® 488 anti-HMGB1	3E8	ICC
Alexa Fluor® 647 anti-HMGB1	3E8	ICC
Ultra-LEAF™ Purified anti-HMGB1	3E8	WB
Direct-Blot™ HRP anti-HMGB1	3E8	WB
Brilliant Violet 421™ anti-HMGB1	3E8	ICFC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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