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PE anti-human C5L2 Antibody

Pricing & Availability
Clone
1D9-M12 (See other available formats)
Regulatory Status
RUO
Other Names
GPR77, C5a receptor-like 2, C5a anaphylatoxin chemotactic receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
1D9-M12_PE_050409.jpg
Human peripheral blood granulocytes stained with 1D9-M12 PE
  • 1D9-M12_PE_050409.jpg
    Human peripheral blood granulocytes stained with 1D9-M12 PE
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342403 25 tests £126
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342404 100 tests £247
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Description

C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88), but it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: Western blotting1,3 and blocking2,3. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor. It has been reported that this clone is not effective in immunohistochemistry of formalin-fixed paraffin-embedded sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed
  2. Hao J, et al. 2013. PLoS One 8:e66305. (Block)
  3. Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)
Product Citations
  1. Spaan A, et al. 2015. J Immunol. 195: 1034-1043. PubMed
  2. Derer S, et al. 2015. J Immunol. 195: 5077 - 5087. PubMed
  3. Schmidt T, et al. 2015. PLoS One. 10: 0132703. PubMed
  4. Rigotto G, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Su S et al. 2018. Cell. 172(4):841-856 . PubMed
RRID
AB_2247831 (BioLegend Cat. No. 342403)
AB_2247831 (BioLegend Cat. No. 342404)

Antigen Details

Structure
Seven transmembrane protein, decoy receptor, does not couple to G-proteins.
Distribution

Neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes.

Function
Negative regulation of C5a function.
Ligand/Receptor
C5a, C5a-des-Arg.
Cell Type
Astrocytes, Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Scola AM, et al. 2009. Mol. Immunol. 46:1149
2. Johswich K, et al. 2006. J. Bio. Chem. 281:39088
3. Gerad NP, et al. 2005. J. Bio. Chem. 280:39677
4. Okinaga S, et al. 2003. Biochemistry. 42:9406

Gene ID
27202 View all products for this Gene ID
UniProt
View information about C5L2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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