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PE anti-human CD117 (c-kit) Recombinant Antibody

Pricing & Availability
Clone
QA18A19 (See other available formats)
Regulatory Status
RUO
Other Names
c-kit, stem cell factor receptor, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA18A19_PE_CD117_Antibody_120722
Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with anti-human CD117 (c-kit) recombinant (clone QA18A19) PE (closed histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • QA18A19_PE_CD117_Antibody_120722
    Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with anti-human CD117 (c-kit) recombinant (clone QA18A19) PE (closed histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
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332203 25 tests £76
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332204 100 tests £169
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cell factor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA18A19 antibody can completely block the binding of clone 104D2 on target cells.

RRID
AB_2936692 (BioLegend Cat. No. 332203)
AB_2936692 (BioLegend Cat. No. 332204)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Growth factor receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
c-Kit ligand
Cell Type
Hematopoietic stem and progenitors, Leukemia, Mast cells
Biology Area
Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Giebel LB, et al. 1992. Oncogene. 7:2207-17.
  2. Furitsu T, et al. 1993. J Clin Invest. 92:1736-44.
Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/07/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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