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PE anti-human CD166 Antibody

Pricing & Availability
Clone
3A6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
CD6 ligand, Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_3A6_PE_082509
PHA-stimulated human peripheral blood lymphocytes (3 days) stained with 3A6 PE
  • A_3A6_PE_082509
    PHA-stimulated human peripheral blood lymphocytes (3 days) stained with 3A6 PE
  • B_3A6_PE_082509
    Human peripheral blood monocytes stained with 3A6 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
343903 25 tests £96
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343904 100 tests £193
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Description

CD166, also known as the CD6 ligand or the Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a 100-105 kD transmembrane glycoprotein. It belongs to the Ig superfamily of proteins and expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently CD166 has also been used as a potential cancer stem cell marker. The antibody reacts with human activated leukocyte cell adhesion molecule (ALCAM).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cultured human thymic epithelial cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections and immunofluorescence.1

Application References

(PubMed link indicates BioLegend citation)
  1. Pretzel D, et al. 2011. Arthritis Res. Ther. 13:R64. (IHC, IF, FC)
Product Citations
  1. He Y, et al. 2023. Protein Cell. 14:202. PubMed
  2. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  3. Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed
  4. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  5. Weeks AM, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  6. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  7. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  8. Argüello RJ, et al. 2012. PLoS One. 7:e35966. PubMed
  9. Morton JJ, et al. 2018. Mol Carcinog. 57:1651. PubMed
  10. Liang C, et al. 2021. Cell Res. 31:187. PubMed
  11. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  12. Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed
  13. Wu CL, et al. 2021. Nat Commun. 12:362. PubMed
  14. Morton JJ, et al. 2020. Molecular Cancer Research. 19(2):346-357. PubMed
  15. Bi S, et al. 2020. Protein Cell. 0.79375. PubMed
  16. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  17. Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed
  18. Ayhan S, et al. 2021. Journal of Cell Science. 134(6):. PubMed
  19. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  20. Xu L, et al. 2016. Cancer Res . 76(23):6901-6910. PubMed
  21. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  22. Duclos GE, et al. 2019. Sci Adv. 5:eaaw3413. PubMed
  23. Fernandez HR, et al. 2018. Cell Death Differ. 25:1239. PubMed
  24. Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed
  25. Fu L, et al. 2019. PLoS Biol. 17:e3000201. PubMed
  26. Thiel A, et al. 2015. Sci Rep. 5: 17685. PubMed
RRID
AB_2289302 (BioLegend Cat. No. 343903)
AB_2289302 (BioLegend Cat. No. 343904)

Antigen Details

Structure
A type I transmembrane glycoprotein belonging to the Ig superfamily of proteins.
Distribution

CD166 is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, and neurons.

Function
Play an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development.
Ligand/Receptor
CD6
Cell Type
Epithelial cells, Fibroblasts, Mesenchymal Stem Cells, Monocytes, Neurons, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Aruffo A, et al. 1997. Immunol Today. 18(10):498
2. Patel DD, et al. 1995. J. Exp. Med. 181:2213
3. Bowen MA, et al. 1995. J. Exp. Med. 181:1563
4. Horst D, et al. 2009. Cancer Invest. 22:1

Gene ID
214 View all products for this Gene ID
UniProt
View information about CD166 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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