PE anti-human CD180 RP105 Antibody anti-CD180

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Clone: MHR73-11 (See other available formats)
Regulatory Status: RUO
Other Names: CD180, Radio-protective 105-kD protein, RP105
Isotype: Mouse IgG1, κ
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Product Citations: publications

MHR73-11_PE_071504 — Human peripheral blood lymphocytes stained with MHR73-11 PE

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312906

100 tests

£189

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Description

CD180, also known as RP105, is a 105 kD member of the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages and dendritic cells. The TLR family's structural characteristics are critically involved in protein-protein signaling interactions leading to the activation of the innate immune system. Additionally, MD-1, a secretory protein, associates with CD180 (RP105) at the cell surface and this complex, in conjunction with TLR4, recognizes bacterial LPS. The human CD180 (RP105) gene maps to 5q12. The MHR73-11 antibody is able to activate B cells and to protect against irradiation- or dexamethasone-induced apoptosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, African Green
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human RP105 complexed with mouse MD-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular staining¹, immunoprecipitation¹, immunohistochemistry¹ of acetone-fixed frozen tissue sections, B cell activation¹, protection from apoptosis induced by radiation or dexamethasone, and induction of the costimulatory molecule CD80 (B7-1)¹. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 312913 and 312914).
RRID: AB_2259841 (BioLegend Cat. No. 312906)

Antigen Details

Structure: Member of the toll-like receptor family, 105 kD
Distribution: B cells, monocytes/macrophages, and dendritic cells
Function: Signaling protein involved in innate immunity
Ligand/Receptor: LPS
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Miura Y, et al. 1998. Blood 92:2815.
Gene ID: 4064 View all products for this Gene ID
UniProt: View information about CD180 on UniProt.org

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Description	Clone	Applications

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Related FAQs

What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All CD180 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD180 (RP105)	MHR73-11	FC,ICFC,IHC-F,IP
PE anti-human CD180 (RP105)	MHR73-11	FC
PE/Cyanine7 anti-human CD180 (RP105)	MHR73-11	FC
APC anti-human CD180 (RP105)	MHR73-11	FC
Ultra-LEAF™ Purified anti-human CD180 (RP105)	MHR73-11	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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