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PE anti-human CD181 (CXCR1) Antibody

Pricing & Availability
Clone
8F1/CXCR1 (See other available formats)
Regulatory Status
RUO
Other Names
CD181, CXCR1, CDw128, CD128a, IL-8RA, Type I IL-8 receptor
Isotype
Mouse IgG2b, κ
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Product Citations
publications
8F1slash-CXCR1-PE_111605
Human peripheral blood granulocytes stained with 8F1 PE
  • 8F1slash-CXCR1-PE_111605
    Human peripheral blood granulocytes stained with 8F1 PE
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320608 100 tests £184
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Description

CXCR1 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor A (IL-8RA), CD181, and CDw128a. It is a CXC chemokine receptor that belongs to the G protein-coupled receptor (CPCR) family. CXCR1 is expressed as homodimer or heterodimer with CXCR2 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. The expression level of CXCR1 on monocytes is lower than that of CXCR2. CXCR1 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α(CXCL1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CXCR1 transfected L1.2 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The 8F1/CXCR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR1 expression.

Product Citations
  1. Brandau S, et al. 2011. J Leukoc Biol. 89:311. PubMed
  2. Silvin A, et al. 2020. Cell. 182:1401. PubMed
  3. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  4. Shin M, et al. 2015. J Immunol. 195: 2861-2869. PubMed
RRID
AB_439815 (BioLegend Cat. No. 320608)

Antigen Details

Structure
Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR2
Distribution

Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes

Function
neutrophil activation, chemotaxis, angiogenesis
Ligand/Receptor
IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-a(CXCL1)
Cell Type
Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M, G. Nilsson. 2005. Chem. Immunol. Allergy. 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164

Gene ID
3577 View all products for this Gene ID
UniProt
View information about CD181 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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