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PE anti-human CD186 (CXCR6) Antibody

Pricing & Availability
Clone
K041E5 (See other available formats)
Regulatory Status
RUO
Other Names
BONZO, STRL33, TYMSTR
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
K041E5_PE_CD186_Antibody_FC_1_121012
Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 APC and CD186 (clone K041E5) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • K041E5_PE_CD186_Antibody_FC_1_121012
    Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 APC and CD186 (clone K041E5) PE (top) or mouse IgG2a, κ PE isotype control (bottom).
  • K041E5_PE_CD186_Antibody_FC_2_121012
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356003 25 tests £109
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356004 100 tests £238
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Description

CD186, also known as CXCR6, is a 39 kD, G-protein coupled chemokine receptor, with seven transmembrane-spanning regions. CXCR6 is expressed on activated and memory T cells. Its ligand is the chemokine CXCL16. CXCR6 is involved in the recruitment of cells to inflamed sites and is also a coreceptor for HIV.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
cDNA encoding human CXCR6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Stegmann K, et al. 2016. Sci Rep. 6: 26157. PubMed
  2. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  3. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  4. Shin M, et al. 2015. J Immunol. 195: 2861-2869. PubMed
  5. Lee CH, et al. 2018. Elife. 7:e32532. PubMed
RRID
AB_2561739 (BioLegend Cat. No. 356003)
AB_2561739 (BioLegend Cat. No. 356004)

Antigen Details

Structure
GPCR, seven transmembrane-spanning regions, 39 kD
Distribution

Memory T cells, activated T cells

Function
Chemotaxis, coreceptor for HIV
Ligand/Receptor
CXCL16
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kim CH, et al. 2001. J. Clin. Invest. 107:595.
2. Nanki T, et al. 2005. Arthritis Rheum. 52:3004.
3. Van der Voort R, et al. 2005. Arthritis Rheum. 52:1381.
4. Aust G, et al. 2005. Eur. J. Endocrinol. 152:635.
5. Agostini C, et al. 2005. Am. J. Respir. Crit. Care Med. 172:1290.
6. Gaida MM, et al. 2008. Clin. Exp. Immunol. 154:216.
7. Huang Y, et al. 2008. J. Immunol. 180:2367.

Gene ID
10663 View all products for this Gene ID
UniProt
View information about CD186 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 12/12/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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