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PE anti-human CD1a Antibody

Pricing & Availability
Clone
HI149 (See other available formats)
Regulatory Status
RUO
Workshop
V CD01.01
Other Names
T6, R4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI149
Molt-4 (T leukemia cell line) stained with HI149 PE
  • HI149
    Molt-4 (T leukemia cell line) stained with HI149 PE
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300105 25 tests £74
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300106 100 tests £163
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Description

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Patton KM, et al. 2005. Infect. Immun.73:2083. PubMed
  4. Curti A, et al. 2010. Haematologica. 95:2022. PubMed
Product Citations
  1. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  2. Schrøder M, et al. 2016. PLoS One. 11: 0157387. PubMed
  3. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  4. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  5. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
RRID
AB_314019 (BioLegend Cat. No. 300105)
AB_314019 (BioLegend Cat. No. 300106)

Antigen Details

Structure
Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution

Cortical thymocytes, Langerhans cells, dendritic cells

Function
Antigen presentation, lymphocyte activation, thymic T cell development
Ligand/Receptor
CD1-restricted TCRs
Cell Type
Dendritic cells, Langerhans cells, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References
  1. Blumberg RS, et al. 1995. Immunol. Rev. 147:5.
  2. Calabi F, et al. 1991. Tissue Antigens 37:1.
  3. Melian A, et al. 1996. Curr. Opin. Immunol. 8:82.
Gene ID
909 View all products for this Gene ID
UniProt
View information about CD1a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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