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PE anti-human CD230 (Prion) Antibody

Pricing & Availability
Clone
3F4 (See other available formats)
Regulatory Status
RUO
Other Names
Major prion protein, p27-30, CD230 antigen, prion protein PrP, prion-related protein
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
3F4_PE_CD230Prion_Antibody_121715
Human peripheral blood lymphocytes were stained with CD230 (clone 3F4) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • 3F4_PE_CD230Prion_Antibody_121715
    Human peripheral blood lymphocytes were stained with CD230 (clone 3F4) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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800317 25 tests £92
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800318 100 tests £205
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Description

Prions cause neurodegenerative disease by aggregating extracellularly within the central nervous system which disrupt the normal tissue structure. This disruption is characterized by "holes" in the tissue with resultant spongy architecture. Two conformational isoforms exist, the normal cellular isoform (PrPC) and the infectious, scrapie isoform (PrPSC). Other histological changes include astrogliosis and the absence of an inflammatory reaction. Neurodegenerative symptoms can include convulsions, dementia, ataxia (balance and coordination dysfunction), and behavioral or personality changes.

All known prion diseases are collectively called transmissible spongiform encephalopathies (TSEs). Prion (PrP) is highly conserved through mammals and comparison between primates ranges from 92.9-99.6% similarity in amino acid sequence. The human protein structure consists of a globular domain with three α-helices and a two-strand antiparallel β-sheet, an NH2-terminal tail, and a short COOH-terminal tail. A glycosylphosphatidylinositol (GPI) membrane anchor at the COOH-terminal tethers PrP to cell membranes. This anchor is integral to the transmission of conformational change; secreted PrP lacking the anchor component is unaffected by the infectious isoform. PrPSC accumulates in compact, protease-resistant aggregates within neural tissue and has a different secondary and tertiary structure from PrPC, but an identical primary sequence.

The primary sequence of PrP is 253 amino acids long before posttranslational modification. Signal sequences in the amino- and carboxy- terminal ends are removed posttranslationally, resulting in a mature length of 208. For human and Syrian hamster PrP, two glycosylated sites exist on helices 2 and 3 at Asn181 and Asn197. Murine PrP has glycosylation sites as Asn180 and Asn196. A disulfide bond exists between Cys179 of the second helix and Cys214 of the third helix (human PrPC numbering).

The precise function of PrP is not yet known, but it is possibly involved in the transport of ionic copper to cells from the surrounding environment. Researchers have also proposed roles for PrP in cell signaling or in the formation of synapses.

Spatial learning, a predominantly hippocampal-function, is decreased in PrP null mice and can be recovered with the reinstatement of PrP in neurons; indicating that loss of PrP function is the cause. PrP is present in both pre- and post-synaptic neuron cells, and the greatest concentration is in the pre-synaptic cells. Some research indicates PrP involvement in neuronal development, differentiation, and neurite outgrowth. The PrP-activated signal transduction pathway is associated with axon and dendritic outgrowth with a series of kinases.

Though most attention is focused on PrP’s presence in the nervous system, it is also abundant in immune system tissue. PrP immune cells include haematopoietic stem cells, mature lymphoid and myeloid compartments, and certain lymphocytes; also, it has been detected in natural killer cells, platelets, and monocytes. T cell activation is accompanied by a strong up-regulation of PrP, though it is not requisite. The lack of immuno-response to transmissible spongiform encephalopathies (TSE), neurodegenerative diseases caused by prions, could stem from the tolerance for PrPSc.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoblotting, immunohistochemistry (IHC) on unfixed tissues, immunoprecipitation (IP) and ELISA.

3F4 is reactive to amino acid residues 109-112 of prion protein (PrP) from humans, hamsters, and felines. It recognizes both protease sensitive and protease resistant forms of PrP (after denaturing).

Application References

(PubMed link indicates BioLegend citation)
  1. Kondoh G, et al. 2005. Nat. Med. 11(2):160-6. (IHC-F)
  2. Gilch S, et al. 2004. Traffic. 5(4):300-13. (WB, IHC-F, ELISA) PubMed
  3. Nishina K, et al. 2004. Biochemistry 43:2613-21. (IP)
  4. Shiga Y, et al. 2004. Neurology 63(3):443-9. (WB, IHC-F, IP, ELISA)
  5. Zou WQ, et al. 2004. Proc. Natl. Acad. Sci. USA 101(5):1380-5.
  6. Kitamoto T, et al. 1987. Lab Invest. 57(2):230-6.
  7. Kascsak RJ, et al. 1987. J. Virol. 61(12):3688-93.
RRID
AB_2616655 (BioLegend Cat. No. 800317)
AB_2616655 (BioLegend Cat. No. 800318)

Antigen Details

Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Prion (CD230)
Antigen References

1. Asante EA, et al. 2015. Nature 522:478.
2. Mead S, et al. 2013. N. Engl. J. Med. 369:1904.
3. Chakrabarti O, Hegde RS. 2009. Cell 137:1136.
4. Wadsworth JD, et al. 2004. Science 306:1793.

Gene ID
5621 View all products for this Gene ID
UniProt
View information about CD230 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10/12/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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