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PE anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
W20111B (See other available formats)
Regulatory Status
RUO
Other Names
2B4, SLAMF4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20111B_PE_CD244_Antibody_072324
Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) Alexa Fluor® 700 and anti-human CD244 (2B4) (clone W20111B) PE (left) or rat IgG2a, κ PE isotype control (right).
  • W20111B_PE_CD244_Antibody_072324
    Human peripheral blood lymphocytes were stained with anti-human CD56 (clone HCD56) Alexa Fluor® 700 and anti-human CD244 (2B4) (clone W20111B) PE (left) or rat IgG2a, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
335205 25 tests £62
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335206 100 tests £137
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Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_3662228 (BioLegend Cat. No. 335205)
AB_3662228 (BioLegend Cat. No. 335206)

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Lee KM, et al. 2003. J Immunol. 170:4881-5.
  2. Chlewicki LK, et al. 2008. J Immunol. 180:8159-67.
  3. Messmer B, et al. 2006. J Immunol. 176:4646-50.
  4. Boles KS, et al. 2001. Immunol Rev. 181:234-49.
Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/15/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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