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PE anti-human CD253 (TRAIL) Antibody

Pricing & Availability
Clone
RIK-2 (See other available formats)
Regulatory Status
RUO
Other Names
Apo-2 ligand, Apo-2L, TNFSF10, CD253
Isotype
Mouse IgG1, κ
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Product Citations
publications
RIK-2_PE_072007
Human TRAIL transfected cells stained with RIK-2 PE
  • RIK-2_PE_072007
    Human TRAIL transfected cells stained with RIK-2 PE
Compare all formats See PE spectral data
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308205 25 tests £89
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308206 100 tests £208
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Description

TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. This antibody is useful for flow cytometric staining and blocking studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TRAIL-transfected mouse cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308214).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
  2. Uno K, et al. 2003. Blood 101:3658. (Block)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  4. Denny MF, et al. 2007. Blood 110:2907. (Block)
  5. Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
Product Citations
  1. Golinelli G, et al. 2021. Transl Oncol. 15:101240. PubMed
  2. Lin CP, et al. 2023. EMBO J. 42:e111614. PubMed
  3. Ribeiro MS, et al. 2023. Methods Mol Biol. 2618:289. PubMed
  4. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
  5. Kalb M, et al. 2012. J Immunol. 188:1583. PubMed
  6. Knörck A, et al. 2022. Front Immunol. 13:838484. PubMed
  7. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  8. Alonso R, et al. 2022. Blood Adv. :. PubMed
  9. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  10. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  11. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
  12. Ray JE, et al. 2021. Cancer Biol Ther. 22:554. PubMed
  13. Lulla AR, et al. 2022. Am J Cancer Res. 12:315. PubMed
  14. Choi Y, et al. 2015. Gut. 64: 1303-1313. PubMed
  15. Achard C, et al. 2017. Oncoimmunology. 6:e1261240. PubMed
RRID
AB_2205825 (BioLegend Cat. No. 308205)
AB_2205825 (BioLegend Cat. No. 308206)

Antigen Details

Structure
TNF ligand superfamily member, approximately 30 kD
Distribution

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Function
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
Ligand/Receptor
DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.

Gene ID
8743 View all products for this Gene ID
UniProt
View information about CD253 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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