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PE anti-human CD27 Antibody

Pricing & Availability
Clone
M-T271 (See other available formats)
Regulatory Status
RUO
Workshop
V 5T CD27.03
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a.M-T271_PE_CD27_Antibody_FC_021113
Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
  • a.M-T271_PE_CD27_Antibody_FC_021113
    Human peripheral blood lymphocytes were stained with CD27 (clone M-T271) PE (filled histogram) or mouse IgG1 PE isotype control (open histogram).
  • b.M-T271_PE_CD27_Antibody__050222
    Human peripheral blood was stained with CD27 (clone M-T271) PE (solid line) or mouse IgG1, κ PE isotype control (dashed line).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
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356405 25 tests £53
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356406 100 tests £139
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T cells from a T-ALL patient.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections1, immunofluorescent staining2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Ma S, et al. 2011. J. Virol. 85:165. (IHC)
  2. Manzo A, et al. 2008. Arthritis Rheum. 11:3377. (IF)
  3. Kato K, et al. 2007. Exp. Hematol. 35:434. (ELISA)
Product Citations
  1. Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed
  2. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  3. Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed
  4. Carre C, et al. 2021. iScience. 24:102970. PubMed
  5. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  6. Schneider MM, et al. 2022. Life Sci Alliance. 5: . PubMed
  7. He T, et al. 2022. Liver Int. 42:1287. PubMed
  8. Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed
  9. He T, et al. 2023. Immun Inflamm Dis. 11:e759. PubMed
  10. Zhang S, et al. 2023. J Clin Med. 12: . PubMed
  11. Li H, et al. 2022. Front Immunol. 13:988004. PubMed
  12. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  13. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  14. Kaku Y, et al. 2021. Cell Reports. 36(2):109385. PubMed
  15. Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed
  16. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  17. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  18. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  19. Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
  20. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  21. Nguyen J, et al. 2021. Mol Syst Biol. 17:e10560. PubMed
  22. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
  23. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  24. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
RRID
AB_2561824 (BioLegend Cat. No. 356405)
AB_2561824 (BioLegend Cat. No. 356406)

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
Distribution

Medullary thymocytes, T and B cell subsets, NK cells

Interaction
CD27 binds to CD70
Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Hintzen R, et al. 1994. Immunol. Today 15:307.
4. Agematsu K, et al. 1995. J. Immunol. 154:3627.

Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/13/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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