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PE anti-human CD34 Antibody

Pricing & Availability
Clone
561 (See other available formats)
Regulatory Status
RUO
Other Names
Gp105-120, My10, Mucosialin
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A_561_PE_072909.jpg
Human peripheral blood mononuclear cells stained with 561 PE (lower panel) or PE mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • A_561_PE_072909.jpg
    Human peripheral blood mononuclear cells stained with 561 PE (lower panel) or PE mouse IgG2a isotype control (upper panel) and CD45 (HI30) PerCP (gated on CD14- cell population)
  • B_561_PE_072909.jpg
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Cat # Size Price Quantity Check Availability Save
343605 25 tests £92
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343606 100 tests £200
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.

Application References

(PubMed link indicates BioLegend citation)
  1. Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82.
  2. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations
  1. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  2. Castañeda-Partida L, et al. 2022. Discov Oncol. 13:28. PubMed
  3. Allouch A, et al. 2022. Nat Commun. 13:6739. PubMed
  4. Hu X, et al. 2023. Hematol Rep. 15:91. PubMed
  5. Che L, et al. 2023. Foods. 12:. PubMed
  6. Wang H, et al. 2023. Mol Ther Methods Clin Dev. 29:213. PubMed
  7. Liu Y, et al. 2023. Int J Stem Cells. . PubMed
  8. Shi T, et al. 2023. J Clin Invest. . PubMed
  9. Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed
  10. Gong Y, et al. 2021. Aging (Albany NY). 13:20629. PubMed
  11. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  12. Boutin J, et al. 2021. Nat Commun. 12:4922. PubMed
  13. Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed
  14. Di Stefano B, et al. 2019. Cell Stem Cell. 25:622. PubMed
  15. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  16. Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed
  17. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  18. Zhang Y, et al. 2018. Stem Cells Int. 2018:7159465. PubMed
  19. Jackson R, et al. 2020. Viruses. 12:00. PubMed
  20. Cui D, et al. 2021. Front Cell Dev Biol. 9:656867. PubMed
  21. Cai S, et al. 2022. Cell Biosci. 12:115. PubMed
  22. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  23. Yao X, et al. 2022. Bioeng Transl Med. 7:e10250. PubMed
  24. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  25. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  26. Yu Y, et al. 2017. Stem Cell Reports. 10.1016/j.stemcr.2017.07.016. PubMed
  27. Niu C, et al. 2017. Mol Med Rep. 16:4879. PubMed
RRID
AB_1732033 (BioLegend Cat. No. 343605)
AB_1732033 (BioLegend Cat. No. 343606)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selecin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 11/01/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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