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PE anti-human CD62E Recombinant Antibody

Pricing & Availability
Clone
QA20A90 (See other available formats)
Regulatory Status
RUO
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A90_PE_CD62E_Antibody_080822
TNF-α stimulated human endothelial cells (HUVEC) stained with anti-human CD62 (clone QA20A90) PE (filled histogram), or PE mouse IgG1, κ isotype control (open histogram).
  • QA20A90_PE_CD62E_Antibody_080822
    TNF-α stimulated human endothelial cells (HUVEC) stained with anti-human CD62 (clone QA20A90) PE (filled histogram), or PE mouse IgG1, κ isotype control (open histogram).
See PE spectral data
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384103 25 tests £79
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384104 100 tests £172
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and be useful for flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA20A90 completely blocks the binding of reference clone HCD62E on target cells.

RRID
AB_2924587 (BioLegend Cat. No. 384103)
AB_2924587 (BioLegend Cat. No. 384104)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression); can also be expressed on endothelial cells in the skin, bone marrow and placenta.

Function
Involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand/Receptor
Siayl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Antigen References
  1. Collins T, et al. 1991. J Biol Chem. 266:2466-73.
  2. Bevilacqua MP, et al. 1987. Proc Natl Acad Sci. USA 84:9238-42.
  3. Berg EL, et al. 1991. J Exp Med. 174:1461-6.
  4. Lawrence MB, et al. 1993. J Immunol. 151:6338-46.
  5. Walz G, et al. 1990. Science. 250:1132-5.
Gene ID
6401 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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