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PE anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK1_PE_112409
Human peripheral blood lymphocytes stained with SK1 PE.
  • SK1_PE_112409
    Human peripheral blood lymphocytes stained with SK1 PE.
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
344705 25 tests £91
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344706 100 tests £223
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Chimpanzee, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Le Y, et al. 2022. iScience. 25:105426. PubMed
  2. Mohamed H, et al. 2023. Biomedicines. 11: . PubMed
  3. Yang Y, et al. 2023. Cancers (Basel). 15:. PubMed
  4. Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed
  5. Li J, et al. 2023. Sci Rep. 13:7501. PubMed
  6. Changsheng H, et al. 2023. Sci Adv. 9:eade4186. PubMed
  7. Landry LG, et al. 2021. Bio Protoc. 11:e3883. PubMed
  8. Zhang H, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  9. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  10. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  11. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  12. Della–Torre E, et al. 2018. Arthritis Rheumatol. 70:1133. PubMed
  13. Vandooren J, et al. 2021. Front Immunol. 12:701739. PubMed
  14. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  15. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  16. Meng W,et al. 2017. Antimicrob Agents Chemother.. 10.1128/AAC.01719-17. PubMed
  17. Wang N, et al. 2021. iScience. 24:103205. PubMed
  18. Price A, et al. 2016. Proc Natl Acad Sci U S A. 113: 10678 - 1068. PubMed
  19. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  20. Ng CY, et al. 2022. EMBO Rep. 23:e54271. PubMed
  21. You L, et al. 2020. Front Immunol. 1.699305556. PubMed
  22. Gimenez-Rivera V, et al. 2016. J Immunol. 197(11):4240-4246. PubMed
  23. Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
  24. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
  25. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  26. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  27. Amer HT, et al. 2022. Front Mol Biosci. 9:1032517. PubMed
  28. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  29. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  30. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  31. Ng CY, et al. 2022. EMBO Rep. 23:e55871. PubMed
  32. Chen J, et al. 2021. J Invest Dermatol. 787:141. PubMed
  33. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  34. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
  35. Wakeley ME, et al. 2020. J Surg Res. 245:610. PubMed
  36. Lee IK, et al. 2020. Cancers (Basel). 12:. PubMed
RRID
AB_1953243 (BioLegend Cat. No. 344705)
AB_1953243 (BioLegend Cat. No. 344706)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD8 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 647 anti-human CD8 SK1 FC
Brilliant Violet 650™ anti-human CD8 SK1 FC
Purified anti-human CD8 SK1 FC,IHC-F
FITC anti-human CD8 SK1 FC
PE anti-human CD8 SK1 FC
PerCP anti-human CD8 SK1 FC
PerCP/Cyanine5.5 anti-human CD8 SK1 FC
PE/Cyanine7 anti-human CD8 SK1 FC
APC/Cyanine7 anti-human CD8 SK1 FC
Alexa Fluor® 488 anti-human CD8 SK1 FC,SB
Pacific Blue™ anti-human CD8 SK1 FC
Biotin anti-human CD8 SK1 FC
APC anti-human CD8 SK1 FC
Alexa Fluor® 700 anti-human CD8 SK1 FC
Purified anti-human CD8 (Maxpar® Ready) SK1 FC,CyTOF®
Brilliant Violet 510™ anti-human CD8 SK1 FC
Brilliant Violet 711™ anti-human CD8 SK1 FC
Brilliant Violet 785™ anti-human CD8 SK1 FC
Brilliant Violet 605™ anti-human CD8 SK1 FC
PE/Dazzle™ 594 anti-human CD8 SK1 FC
PE anti-human CD8 SK1 FC
APC/Fire™ 750 anti-human CD8 SK1 FC
APC anti-human CD8 SK1 FC
Brilliant Violet 421™ anti-human CD8 SK1 FC
Pacific Blue™ anti-human CD8 SK1 FC
TotalSeq™-A0046 anti-human CD8 SK1 PG
TotalSeq™-C0046 anti-human CD8 SK1 PG
Brilliant Violet 750™ anti-human CD8 SK1 FC
TotalSeq™-B0046 anti-human CD8 SK1 PG
Spark Blue™ 550 anti-human CD8 SK1 FC
PE/Cyanine7 anti-human CD8 SK1 FC
FITC anti-human CD8 SK1 FC
APC/Fire™ 810 anti-human CD8 SK1 FC
PE/Fire™ 640 anti-human CD8 SK1 FC
PE/Fire™ 700 anti-human CD8 SK1 FC
PerCP anti-human CD8 SK1 FC
PerCP/Cyanine5.5 anti-human CD8 SK1 FC
TotalSeq™-D0046 anti-human CD8 SK1 PG
APC/Fire™ 750 anti-human CD8 SK1 FC
GMP APC anti-human CD8 SK1 FC
PE/Cyanine5 anti-human CD8 Antibody SK1 FC
Spark UV™ 387 anti-human CD8 SK1 FC
GMP PE anti-human CD8 SK1 FC
GMP PE/Cyanine7 anti-human CD8 SK1 FC
Spark NIR™ 685 anti-human CD8 SK1 FC
KIRAVIA Blue 520™ anti-human CD8 SK1 FC
GMP FITC anti-human CD8 SK1 FC
GMP Pacific Blue™ anti-human CD8 SK1 FC
GMP PerCP anti-human CD8 SK1 FC
Spark Violet™ 500 anti-human CD8 SK1 FC
GMP APC/Fire™ 750 anti-human CD8 SK1 FC
GMP PerCP/Cyanine 5.5 anti-human CD8 SK1 FC
Alexa Fluor® 660 anti-human CD8a SK1 FC
Spark Blue™ 515 anti-human CD8 SK1 FC
Spark Blue™ 574 anti-human CD8 SK1 FC
Spark Violet™ 538 anti-human CD8 SK1 FC
PE/Fire™ 810 anti-human CD8 SK1 FC
Spark YG™ 593 anti-human CD8 SK1 FC
PerCP/Fire™ 806 anti-human CD8 SK1 FC

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Go To Top Version: 4    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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