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PE anti-human EOS Antibody

Pricing & Availability
Clone
W16032A (See other available formats)
Regulatory Status
RUO
Other Names
Ikaros family zinc finger protein 4 (IKZF4), ZMFN1A4, KIAA1782
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16032A_PE_EOS_Antibody_1_062920
Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with EOS PE (clone W16032A) (left) or rat IgG2a, κ PE isotype (right), and FOXP3 Alexa Fluor® 647. Cells shown are gated on CD3+CD4+ cells.
  • W16032A_PE_EOS_Antibody_1_062920
    Human peripheral blood lymphocytes were surface stained with CD3 FITC and CD4 APC/Fire™ 750 and then treated with the True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with EOS PE (clone W16032A) (left) or rat IgG2a, κ PE isotype (right), and FOXP3 Alexa Fluor® 647. Cells shown are gated on CD3+CD4+ cells.
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399303 25 tests £105
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399304 100 tests £271
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Description

EOS also known as IKZF4 is an IKAROS Family Member expressed in lymphocytes, specifically in Tregs. It plays an important role in the inhibitory function of Tregs. It may be involved in the development of the central and peripheral nervous systems as well as lymphoid development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EOS recombinant protein (271-520a.a)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876723 (BioLegend Cat. No. 399303)
AB_2876723 (BioLegend Cat. No. 399304)

Antigen Details

Structure
IKAROS Family Member
Distribution

Lymphocytes, specifically in Tregs

Function
Essential for inhibitory function of Tregs
Interaction
Binds to 5'GGGAATRCC-3' Ikaros-binding sequence
Antigen References
  1. Honma Y, et al. 1999. FEBS Lett. 447(1):76-80.
  2. Perdomo J, et al. 2000. J. Biol chem 275(49):38347-54.
  3. Westman BJ, et al. 2004. Biochemistry. 43(42):13318-27.
  4. Pan F, et al. 2009. Science. 325(5944):1142-6.
Gene ID
64375 View all products for this Gene ID
UniProt
View information about EOS on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/29/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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