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PE anti-human erbB3/HER-3 Antibody

Pricing & Availability
Clone
1B4C3 (See other available formats)
Regulatory Status
RUO
Other Names
erbB-3, erbB3, HER-3, HER3
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1B4C3_PE_111407
Human breast cancer cell line MCF-7 stained with 1B4C3 PE
  • 1B4C3_PE_111407
    Human breast cancer cell line MCF-7 stained with 1B4C3 PE
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324705 25 tests £92
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324706 100 tests £200
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Description

The 1B4C3 monclonal antibody recognizes human erbB3/HER3 also know as oncogene erbB3, tyrosine kinase cell surface receptor HER3, and C-erbB3. erbB3/HER3 is a receptor tyrosine kinase and a member of the epidermal growth factor receptor family. erbB3/HER3 binds the heregulin ligands and contains 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain with a predicted molecular weight approximately 148 kD. Signaling through the erbB3/HER3 receptor induces proliferation and differentiation in some cell types, the receptor is widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. erbB3/HER3 forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in bladder and ovarian cancer. erbB3/HER3 has been shown to interact with a number of proteins including EGF receptor, erbB2/HER2, erbB4, Grb2, Grb7, SOS1, cdk5, SHC, FAK, PTK6, and others. The erb3/HER3 receptor is extensively phosphorylated at residues T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, and Y1328. The 1B4C3 antibody has been shown to be useful for flow cytometric detection of human erbB3/HER3.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3 cells transfected with human erbB3/HER3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Tiago M, et al. 2020. Br J Cancer. 122:789. PubMed
  2. Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed
  3. Zabransky DJ, et al. 2015. Proc Natl Acad Sci U S A. 112: 6205 -6214. PubMed
  4. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  5. Marrocco I, et al. 2021. EMBO Mol Med. 13:e13144. PubMed
  6. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
RRID
AB_2099569 (BioLegend Cat. No. 324705)
AB_2099569 (BioLegend Cat. No. 324706)

Antigen Details

Structure
Member of the epidermal growth factor receptor family. A receptor tyrosine kinase containing 4 furin-like repeats, 2 cheY receiver domains, and a tyrosine kinase domain. Predicted molecular weight approximately 148 kD
Distribution

Widely expressed in many tissues including kidney, lung, brain, placenta, skin and stomach. Forms constitutively active heterodimers with erbB2/HER2 in a subset of human mammary tumors; expression of erbB3/HER3 reported be linked to favorable outcome in b

Function
Induces extensive signaling cascade that can regulate growth and possibly differentiation
Ligand/Receptor
Heregulin
Modification
Extensively phosphorylated T873 and S1123 (by cdk5); Y1199, Y1262, Y1289, Y1328
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Carraway KL, et al. 1994. J. Biol. Chem. 269:14303.
2. Kraus MH, et al. 1989. Proc. Natl. Acad. Sci. USA 86:9193.
3. Chan SD, et al. 1995. J. Biol. Chem. 270:22608.
4. Tanner B, et al. 2006. J. Clin. Oncol. (Epub Aug. 8 2006)

Gene ID
2065 View all products for this Gene ID
UniProt
View information about erbB3/HER-3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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