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PE anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA16A02 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA16A02_PE_GranzymeB_Antibody_1_042817
Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B PE (clone QA16A02, top) or mouse IgG1, κ PE isotype control (bottom).
  • QA16A02_PE_GranzymeB_Antibody_1_042817
    Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B PE (clone QA16A02, top) or mouse IgG1, κ PE isotype control (bottom).
  • QA16A02_PE_GranzymeB_Antibody_2_042817
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372207 25 tests £89
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372208 100 tests £230
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  2. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  3. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  4. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  5. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  6. Lin J, et al. 2023. Nat Commun. 14:224. PubMed
  7. Jiang X, et al. 2023. Cell Discov. 9:7. PubMed
  8. Chen M, et al. 2023. Cell Biosci. 13:19. PubMed
  9. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  10. Allen SD, et al. 2021. Biomaterials. 269:120635. PubMed
  11. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  12. Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed
  13. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  14. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  15. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  16. Yang Z, et al. 2022. J Immunother Cancer. 10:. PubMed
  17. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  18. Liu X, et al. 2020. Nature. . PubMed
  19. Yang X, et al. 2021. Front Pharmacol. 12:771046. PubMed
  20. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  21. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  22. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  23. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  24. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  25. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  27. Hubrack S, et al. 2021. Sci Rep. 11:14090. PubMed
  28. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  29. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  30. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
  31. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  32. Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed
  33. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  34. Kim S, et al. 2020. Autophagy. :1. PubMed
  35. Li Z, et al. 2020. J Immunol. 2589:204. PubMed
  36. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  37. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  38. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  39. Li ZY, et al. 2021. J Exp Med. 218:. PubMed
  40. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  41. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  42. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  43. Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed
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  45. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
RRID
AB_2687031 (BioLegend Cat. No. 372207)
AB_2687031 (BioLegend Cat. No. 372208)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 05/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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