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PE anti-human P2RY12 Antibody

Pricing & Availability
Clone
S16001E (See other available formats)
Regulatory Status
RUO
Other Names
ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
S16001E_PE_P2RY12_Antibody_082317
Human peripheral blood platelets were stained with P2RY12 (clone S16001E) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
  • S16001E_PE_P2RY12_Antibody_082317
    Human peripheral blood platelets were stained with P2RY12 (clone S16001E) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
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392103 25 tests £105
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392104 100 tests £230
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Description

P2RY12 is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system and is not activated by UDP and UTP.

P2RY12 is required for normal platelet aggregation and blood coagulation, and is a target to inhibit of platelet aggregation in the treatment of thromboembolisms and other clotting disorders. P2RY12 is expressed on platelets, lung, appendix, pituitary and adrenal glands; this molecule is a very useful marker to identify microglial cells in the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human P2RY12-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.

Product Citations
  1. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  2. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  3. Bodnar B, et al. 2021. Front Cell Neurosci. 15:682272. PubMed
  4. Woolf Z, et al. 2021. STAR Protocols. 2(2):100518. PubMed
  5. Scheiblich H, et al. 2021. Cell. 184:5089. PubMed
  6. Palacios-Acedo AL, et al. 2021. Front Oncol. 11:704945. PubMed
RRID
AB_2716006 (BioLegend Cat. No. 392103)
AB_2716006 (BioLegend Cat. No. 392104)

Antigen Details

Distribution

Platelets, microglia

Function
Inhibit the adenylyl cyclase second messenger system
Ligand/Receptor
ADP, ATP
Cell Type
Microglia, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
GPCR, Purinergic Receptors
Antigen References

1. Lecchi A, et al. 2015. Blood. 125:1006.
2. Zhang K, et al. 2014. Nature. 509:115.
3. Zhang J, et al. 2014. Nature. 509:119.
4. Cornelissen I, et al. 2010. Proc. Natl. Acad. Sci. USA. 107:18605.
5. Paruchuri S, et al. 2009. J. Exp. Med. 206:2543.

Gene ID
64805 View all products for this Gene ID
UniProt
View information about P2RY12 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/31/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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