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PE anti-human Siglec-10 Antibody

Pricing & Availability
Clone
5G6 (See other available formats)
Regulatory Status
RUO
Other Names
Sialic acid binding Ig-like lectin 10
Isotype
Mouse IgG1, κ
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Product Citations
publications
5G6_PE_021810
Human peripheral blood lymphocytes stained with anti-human CD19 and 5G6 PE
  • 5G6_PE_021810
    Human peripheral blood lymphocytes stained with anti-human CD19 and 5G6 PE
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347603 25 tests £105
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347604 100 tests £247
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Description

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-10 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Munday J, et al. 2001. Biochem. J. 355:489
  2. Yokoi H, et al. 2006. Allergy. 61:769
  3. Kivi E, et al. 2009. Blood 114:5385
  4. Barkal AA, et al. 2019. Nature. 572(7769):392-396. (Agonist)
Product Citations
  1. Büll C, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.105. PubMed
  2. Wang Z, et al. 2020. Cell Mol Immunol. 17:1148. PubMed
RRID
AB_2270417 (BioLegend Cat. No. 347603)
AB_2270417 (BioLegend Cat. No. 347604)

Antigen Details

Structure
Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution

Monocytes, eosinophils, B cells, and a subset of NK cells

Function
Cell adhesion, signal transduction
Interaction
SHP-1, PTPN6
Ligand/Receptor
CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
B cells, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Siglec Molecules
Antigen References

1. Chen GY, et al. 2009. Science 323:1722
2. Aizawa H, et al. 2003. Genomics.82:521
3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355

Gene ID
89790 View all products for this Gene ID
UniProt
View information about Siglec-10 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 03/29/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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