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PE anti-IRF7 Antibody

Pricing & Availability
Clone
12G9A36 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor-7 (IRF-7)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
12G9A36_PE_IRF7_Antibody_FC_011614
Overnight starved Jurkat cells were treated with or without IFN-α for 6 hours, then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF7 (clone 12G9A36) PE (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ PE isotype control (dash line for unstimulated, dot line for stimulated).
  • 12G9A36_PE_IRF7_Antibody_FC_011614
    Overnight starved Jurkat cells were treated with or without IFN-α for 6 hours, then fixed and permeabilized with FOXP3 Fix/Perm Buffer Set. Cells were then stained with IRF7 (clone 12G9A36) PE (solid line with open histogram for untreated cells, filled histogram for stimulated cells) or mouse IgG2b, κ PE isotype control (dash line for unstimulated, dot line for stimulated).
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656003 25 tests £113
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656004 100 tests £271
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Description

IRF-7 is a member of the interferon regulatory transcription factor family that plays key regulatory roles in innate immunity. Upon virus infection, IRF-7 forms a complex with MyD88 and TRAF6 when stimulated by toll-like receptors TLR7, TLR8, and TLR9, which in turn results in nuclear translocation and induction of interferon gene expression. IRF-7 gene expression is upregulated by the stimulation of interferon through the Jak-STAT signaling pathway, which forms a positive feedback loop. The protein level of IRF-7 after activation is then downregulated by the ubiquitin-proteasome system to avoid excessive production of interferons that may cause damage to the host. Virus induced type I interferon expression was completely abrogated in IRF-7 knockout mice, suggesting that IRF-7 is critical for interferon mediated host defense during virus infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial IRF7 recombinant protein (127-503 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2563007 (BioLegend Cat. No. 656003)
AB_2563007 (BioLegend Cat. No. 656004)

Antigen Details

Structure
503 amino acids, predicted molecular weight of 54 kD, contains an IRF tryptophan pentad repeat responsible for DNA binding
Distribution

Shuttles between the cytoplasm and nucleus; activated (phosphorylated) form of IRF-7 accumulates in the nucleus

Function
Transcription factor that transactivates proinflamatory cytokines and type I interferon genes in response to virus infection
Interaction
Forms homodimer or heterodimer with IRF-3, interacts with TICAM-1, TICAM-2, MyD88, and TRAF6
Cell Type
Dendritic cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Antigen References

1. Owens BM, et al. 2012. PLoS One. 7:e41050.
2. Yu Y, et al. 2010. Immunity. 33:863.
3. Liang Q, et al. 2011. J. Immunol. 186:1001.
4. Buss C, et al. 2010. J. Immunol. 184:3072.
5. Joo CH, et al. 2007. J. Virol. 81:8282.
6. Balachandran S, et al. 2007. J. Immunol. 178:2429.

Gene ID
3665 View all products for this Gene ID
UniProt
View information about IRF7 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/22/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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