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PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
W19132B (See other available formats)
Regulatory Status
RUO
Other Names
SignalingLymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
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Product Citations
publications
W19132B_PE_CD150_Antibody_083022
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD19 APC and anti-mouse CD150 (SLAM) PE (clone W19132B) (left) or rat IgG2a, λ PE isotype control (right).
  • W19132B_PE_CD150_Antibody_083022
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD19 APC and anti-mouse CD150 (SLAM) PE (clone W19132B) (left) or rat IgG2a, λ PE isotype control (right).
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162605 25 µg £62
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162606 100 µg £161
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Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RBL1 transfectant (1-287a.a)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2927864 (BioLegend Cat. No. 162605)
AB_2927864 (BioLegend Cat. No. 162606)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Antigen References

1. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
2. Gordiienko I, et al. 2019. Clin Immunol. 204:14-22
3. Kiel M, et al. 2005. Cell. 121(7):1109-21

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/30/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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