Loading...

PE anti-mouse CD154 Antibody

Pricing & Availability
Clone
MR1 (See other available formats)
Regulatory Status
RUO
Other Names
CD40 ligand (CD40L), gp39, T-BAM, TRAP, Ly-62, TNFSF5
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
MR1
PMA- and ionomycin-stimulated (6 hrs) BALB/c T cells stained with MR1 PE
  • MR1
    PMA- and ionomycin-stimulated (6 hrs) BALB/c T cells stained with MR1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
106505 50 µg £76
Check Availability


Need larger quantities of this item?
Request Bulk Quote
106506 200 µg £204
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Activated mouse Th1 clone D1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 106515-106520).

Application References

(PubMed link indicates BioLegend citation)
  1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
  2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
  3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
  4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
  5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
Product Citations
  1. Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed
  2. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  3. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  4. Gengenbacher M, et al. 2014. MBio. 5:1262. PubMed
  5. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  6. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  7. Danelli L, et al. 2015. Cancer Immunol Res. 3:85. PubMed
  8. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  9. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  10. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  11. Iwata H, et al. 2013. J Immunol. 191:2978. PubMed
  12. Chen C, et al. 2011. PLoS One. 6:e17712. PubMed
  13. Kimura S, et al. 2020. Am J Transplant. 20:977. PubMed
  14. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  15. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed
RRID
AB_313271 (BioLegend Cat. No. 106505)
AB_313271 (BioLegend Cat. No. 106506)

Antigen Details

Structure
TNF superfamily, 39 kD
Distribution

Activated CD4+ T cells

Function
T-B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550.
3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21947 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account