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PE anti-mouse CD178.1 (FasL) Antibody

Pricing & Availability
Clone
Kay-10 (See other available formats)
Regulatory Status
RUO
Other Names
Fas Ligand, FasL, Apo-1 Ligand, CD95 Ligand
Isotype
Mouse IgG2b, κ
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Product Citations
publications
Kay-10
Mouse Fas Ligand transfected cells stained with Kay-10 PE
  • Kay-10
    Mouse Fas Ligand transfected cells stained with Kay-10 PE
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106805 50 µg £89
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Description

CD178.1 is a 40 kD Fas ligand alloantigen (designated as mFasL.1) expressed on the activated T lymphocytes, testis, and eye of selected mouse strains including C57BL/6, C3H, MRL, NOD, NZB, NZW, and SJL. It does not react with lymphoblasts from BALB/c, DBA/1, or DBA/2 mice. CD178.1 is upregulated in activated T cells upon TCR re-engagement and has been shown to induce autocrine and paracrine T cell death. CD178 expression in the eye and testis has been shown to participate in immune privilege at these sites. Functional studies suggest that mFasL.2 has higher specific activity than mFasL.1. CD178 can be cleaved from the surface by metalloproteases, and "soluble" CD178 may block the activities of membrane-bound CD178. CD178 binds to CD95 (Fas) to induce apoptotic cell death implicated in the maintenance of peripheral tolerance. CD178/CD95 interactions have been implicated in the proliferation of CD8+ cells and neutrophil extravasation, chemotaxis, and survival. The Kay-10 antibody has been reported to block CD178.1/CD95 induced apoptosis in cells derived from mice expressing this alloantigen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/6 mouse FasL cDNA-transfected L5178Y cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking1,2 and in vivo blocking3-5, and immunohistochemical staining6 of formalin-fixed paraffin-embedded tissue sections. Fas Ligand is expressed at low density on activated cells. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106806) or by using a high sensitivity, three-layer staining technique (e.g., including biotinylated anti-mouse IgG (Cat. No. 405303) second step , followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106809 and 106810).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.
  2. Kobata T, et al. 1997. Immunology 92:206.
  3. Hattori K, et al. 1998. Blood 91:4051.
  4. Takeda Y, et al. 1998. Diabetologia 41:315.
  5. Kim YH, et al. 1999. Eur. J. Immunol. 29:455.
  6. Takeda Y, et al. 1998. Diabetologia 41:315.
Product Citations
  1. Kwee BJ, et al. 2018. Biomaterials. 109:178. PubMed
  2. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  3. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
RRID
AB_2246643 (BioLegend Cat. No. 106805)

Antigen Details

Structure
TNF superfamily Fas ligand alloantigen, 40 kD
Distribution

Activated T cells, spleen, testis, eye of some selected mouse strains (C57BL/6, C3H, NZB, NZw, MRL)

Function
Induces apoptosis
Ligand/Receptor
Fas (CD95)
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Nagata S. 1999. Annu. Rev. Genet. 33:209.
3. Takahashi T, et al. 1994. Cell 76:969.
4. Hill LL, et al. 1999. Science 285:898.
5. Kayagaki N, et al. 1997. P. Natl. Acad. Sci. USA 94:3914.

Gene ID
14103 View all products for this Gene ID
UniProt
View information about CD178.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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